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Alterations in intracellular calcium transients of fibroblasts from progressive systemic sclerotic patients: a digital imaging microscopic study

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Summary

Intracellular Ca2+ concentrations ([Ca2+]i) in cultured skin fibroblasts from normal subjects and progressive systemic sclerosis (PSS) patients were determined by using Fura-2 and fluorescent videomicroscopy. With the exception of fibroblasts from one PSS patient showing a higher [Ca2+]i, no significant difference was observed in resting [Ca2+]i between the two groups of fibroblasts. Bradykinin (BK) (10 ΜM) induced a transient [Ca2+]i increase in normal fibroblasts, whereas the BK-induced [Ca2+]i increase was reduced or not detectable in PSS fibroblasts. Removal of extracellular Ca2+ did not eliminate the BK-induced [Ca2+]i increase in normal fibroblasts. These findings suggest that BK stimulates Ca2+ release from intracellular stores in human fibroblasts, and also that the BK-mediated Ca2+ release is impaired in PSS fibroblasts.

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Authors and Affiliations

  1. Department of Dermatology, Gifu University School of Medicine, Tsukasamachi-40, 500, Gifu, Japan

    M. Seishima & S. Mori

  2. Department of Neuroscience, Mitsubishi-Kasei Institute of Life Sciences, Machida, Japan

    Y. Kudo

  3. Department of Biochemistry, Gifu University School of Medicine, Gifu, Japan

    S. Nagao & Y. Nozawa

Authors
  1. M. Seishima
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  2. Y. Kudo
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  3. S. Nagao
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  4. S. Mori
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  5. Y. Nozawa
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Seishima, M., Kudo, Y., Nagao, S. et al. Alterations in intracellular calcium transients of fibroblasts from progressive systemic sclerotic patients: a digital imaging microscopic study. Arch Dermatol Res 283, 96–99 (1991). https://doi.org/10.1007/BF00371616

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  • DOI: https://doi.org/10.1007/BF00371616

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