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Immunoelectron microscopic localization of fibronectin in cultured human keratinocytes

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Abstract

We investigated the ultrastructural localization of fibronectin (FN) in cultured human keratinocytes using an improved method of peroxidase-immunoelectron microscopy. This method enabled us to visualize the precise localization of FN within the cells while preserving the morphology. FN was localized in the protein synthetic and secretory organelles, including the rough-surfaced endoplasmic reticulum, Golgi complex, multivesicular bodies and perinuclear space. It was also detected in the extracellular space, on small regions of the villous projections of cell membranes at the site of secretion and at cell-substratum contact sites. These findings confirm that human keratinocytes synthesize, secrete and deposit FN in the pericellular matrix.

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Authors and Affiliations

  1. Department of Dermatology, Shinshu University School of Medicine, 390, Matsumoto, Japan

    M. Kubo & T. Saida

  2. Department of Anatomy, Yamanashi Medical College, 409-38, Yamanashi, Japan

    S. Ohno

  3. Department of Biomedical Food Research, National Institute of Health, 162, Shinjuku-ku, Japan

    M. Kato

  4. School of Food and Nutritional Sciences, University of Shizuoka, 422, Shizuoka, Japan

    M. Isemura

  5. Department of Dermatology, Tohoku University School of Medicine, 980, Sendai, Japan

    H. Tagami

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  1. M. Kubo
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  2. S. Ohno
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  4. M. Isemura
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  5. H. Tagami
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  6. T. Saida
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Kubo, M., Ohno, S., Kato, M. et al. Immunoelectron microscopic localization of fibronectin in cultured human keratinocytes. Arch Dermatol Res 286, 448–454 (1994). https://doi.org/10.1007/BF00371570

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  • DOI: https://doi.org/10.1007/BF00371570

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