Abstract
Atopic dermatitis is a disease with a genetic predisposition affecting the immune system, with T lymphocytes participating in the immune dysregulation. Most in vitro T lymphocyte studies of atopic dermatitis have focused on antigen-specific T-cell clones. However, antigen-non-specific regulatory T lymphocytes may also take part in the pathway leading to antigen-specific clonal T-lymphocyte proliferation. T lymphocytes from skin biopsy specimens from three patients with severe atopic dermatitis were cultured in the presence of IL-2 and IL-4, but without antigen added. Initially, proliferation was oligo- or polyclonal, but in all cases overgrowth by T cells with clonal chromosomal aberrations was subsequently observed. These abnormal T-cell clones demonstrated continuous growth and complete or partial phenotypic loss of the T-cell antigen receptor complex. In summary, these findings suggest that a subset of aberrant skin-homing T lymphocytes is associated with atopic dermatitis.
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References
Bos JD, Wierenga EA, Smitt JHS, van der Heijden FL, Kapsenberg ML (1992) Immune dysregulation in atopic eczema. Arch Dermatol 128: 1509–1512
Cooper KD (1994) Atopic dermatitis: recent trends in pathogenesis and therapy. J Invest Dermatol 102: 128–137
Jensen AW, Hokland M, JØrgensen H, Justesen J, Ellegaard J, Hokland P (1991) Solitary expression of CD7 among T-cell antigens in acute myeloid leukemia: identification of a group of patients with similar T-cell receptor Β and δ rearrangements and course of disease suggestive of poor prognosis Blood 78: 1292–1300
Perillo NL, Walford RL, Newman MA, Effros RB (1989) Human T lymphocytes possess a limited in vitro life span. Exp Gerontol 24: 177–187
Pawelec G, Schneider EM, Wernet P (1986) Acquisition of suppressive activity and natural killer-like cytotoxicity by human alloproliferative “helper” T cell clones. J Immunol 136: 402–411
Reinhold U, Kukel S, Goeden B, Neumann U, Wehrmann W, Kreysel H-W (1991) Interleukin-4 promotes the expansion of skin-infiltrating lymphocytes from atopic dermatitis in vitro. J Invest Dermatol 96: 370–375
Kaltoft K, Bisballe S, Dyrberg T, Boel E, Rasmussen PB, Thestrup-Pedersen K (1992) Establishment of two continuous T-cell strains from a single plaque of a patient with mycosis fungoides. In Vitro Cell Dev Biol 28A: 161–167
Kaltoft K, Bisballe S, Sterry W, SØgaard H, Thestrup-Pedersen K (1994) Sezary syndrome is characterized by genotraumatic T-cells. In: Vloten WA van, Lambert WC (eds) Basic mechanisms of physiologic and aberrant lymhoproliferation in the skin. Plenum Press, New York, pp 401–418
Kaltoft K, Holm Hansen B, Thestrup-Pedersen K (1994) Cytogenetic findings in cell lines from CTCL. Dermatol Clin 12: 295–304
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Kaltoft, K., Pedersen, C.B., Hansen, B.H. et al. In vitro genetically aberrant T-cell clones with continuous growth are associated with atopic dermatitis. Arch Dermatol Res 287, 42–47 (1994). https://doi.org/10.1007/BF00370717
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DOI: https://doi.org/10.1007/BF00370717