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Pflügers Archiv

, Volume 419, Issue 6, pp 644–650 | Cite as

Effects of calcitonin gene-related peptide on membrane currents in mammalian cardiac myocytes

  • Toshiaki Nakajima
  • Reiko Takikawa
  • Tsuneaki Sugimoto
  • Yoshihisa Kurachi
Excitable Tissues and Central Nervous Physiology

Abstract

We examined the effects of calcitonin gene-related peptide (CGRP) on the membrane currents of single atrial and ventricular cells of guinea pig heart. The tightseal whole-cell voltage-clamp technique was used. In atrial cells, like isoproterenol, CGRP increased the L-type Ca channel current (ICa.L) in a concentration-dependent manner. Human CGRP-(8-37), a putative CGRP receptor antagonist, completely abolished the CGRP-induced increase of ICa.L. Although the effects of CRGP were similar to those of isoproterenol, propranolol, a β-adrenergic receptor antagonist, did not affect the CGRP-induced increase of ICa.L. After ICa.L had been maximally activated by isoproterenol (2 μM) or intracellular cyclic adenosine 5′-monophosphate (100 μM), CGRP failed to increase ICa.L. Acetylcholine antagonized the effects of CGRP on ICa.L. Unlike the effects on atrial cells, CGRP had no significant effects on the membrane currents of ventricular myocytes. Thes results indicate that CGRP increases ICa.L via adenylate cyclase activation by binding to specific membrane receptors in cardiac atrial myocytes. Furthermore, CGRP receptors are expressed in atrial cells but probably not in ventricular cells.

Key words

Calcitonin gene-related peptide Calcitonin Single cardiac myocytes Whole-cell voltage-clamp 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Toshiaki Nakajima
    • 1
  • Reiko Takikawa
    • 1
  • Tsuneaki Sugimoto
    • 1
  • Yoshihisa Kurachi
    • 2
  1. 1.The 2nd Department of Internal Medicine, Faculty of MedicineUniversity of TokyoTokyoJapan
  2. 2.Division of Cardiovascular Diseases, Departments of Internal Medicine and PharmacologyMayo Clinic, Mayo FoundationRochesterUSA

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