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Pflügers Archiv

, Volume 419, Issue 6, pp 572–578 | Cite as

The effect of hypo-osmolarity upon transepithelial ion transport in cultured renal epithelial layers (MDCK)

  • N. L. Simmons
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

Regulatory volume decrease after exposure to hypo-osmotic media in MDCK epithelial cells results from activation of both K+ and Cl conductances. Swelling-stimulated 86Rb(K) losses were observed only across the basal-lateral membrane and were relatively insensitive to 10 mM Ba2+. The effect of hypo-osmotic media upon MDCK epithelia mounted in Ussing chambers has been investigated. Exposure of the basal-lateral surfaces to hypo-osmotic media resulted in a transient stimulation of inward short-circuit current (Isc) followed by inhibition of inward Isc in both control layers and in layers where inward current (due to transepithelial Cl secretion) was first stimulated by 5 μM prostaglandin E1 (PGE1). The transient stimulation of inward current by hypo-osmotic media was not markedly attenuated by 10 mM Ba2+ in PGE1-stimulated layers. After stimulation of inward (Cl-secretory) current to high levels by 10μM adrenaline, the predominant effect of basal-lateral exposure to hypo-osmotic media was an inhibition of the inward current. This inhibition was partially reversed by 40μM 4,4′-diisothiocyanatostilbene-2,2′-disulphonate (DIDS). The stimulation, then inhibition, of inward Isc is likely to be the result of separate swelling-induced K+ and Cl conductances (respectively) at the basal-lateral membrane. The swelling-stimulated Cl conductance is distinct from the apical Cl conductance regulated by PGE1 or adrenaline.

Key words

Cell volume regulation MDCK cell Renal epithelial cell Cl secretion 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • N. L. Simmons
    • 1
  1. 1.Department of Physiological SciencesThe Medical SchoolNewcastle upon TyneUK

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