Abstract
We have previously shown that long terminal repeats (LTRs) derived from various isolates of SIVAGM share a unique functional property. In the absence of viral Tat, all SIVAGM LTRs act as much more efficient promoters than any of the other LTRs derived from representative primate immunodeficiency viruses. In the presence of Tat, however, SIVAGM LTRs are activated relatively inefficiently. To map the elements that confer these features on the SIVAGM LTR, a number of deletion mutants were constructed, and their promoter activities were determined using a bacterial CAT gene as a marker. The results obtained indicated that various elements located in the U3 region may contribute to the high basal promoter activity and that no negative elements are present in the region. The Tat-responsive sequence TAR was localized to the R region as observed for the other LTRs. A mutant carrying a single nucleotide deletion in this region completely lost responsiveness to Tat protein.
Similar content being viewed by others
References
SakaiH., SakuragiJ., SakuragiS., KawamuraM., and AdachiA., Arch Virol 129, 1–10, 1993.
DesrosiersR.C., Nature 345, 288–289, 1990.
SakuragiJ., FukasawaM., ShibataR., SakaiH., KawamuraM., AkariH., KiyomasuT., IshimotoA., HayamiM., and AdachiA., Virology 185, 455–459, 1991.
AdachiA., GendelmanH.E., KoenigS., FolksT., WilleyR., RabsonA., and MartinM.A., J Virol 59, 284–291, 1986.
KikukawaR., KoyanagiY., HaradaS., KobayashiN., HatanakaM., and YamamotoN., J Virol 57, 1159–1162, 1986.
SakaiH., FurutaR.A., TokunagaK., KawamuraM., HatanakaM., and AdachiA., FEBS Lett 365, 141–145, 1995.
WiglerM., PellicerA., SilversteinS., AxelR., UrlaubG., and ChasinL., Proc Natl Acad Sci USA 76, 1373–1376, 1979.
TakaiT., OhmoriH., Biochim Biophys Acta 1048, 105–109, 1990.
GormanC.M., MoffatL.F., and HowardB.H., Mol Cell Biol 2, 1044–1051, 1982.
HaseltineW.A. in HaseltineW.A. and Wong-StaalF. (eds.) Genetic Structure and Regulation of HIV, Raven Press, New York, 1991, pp 1–42.
RosenC.A., SodroskiJ.G., and HaseltineW.A., Cell 41, 813–823, 1985.
DingwallC., ErnbergI., GaitM.J., GreenS.M., HeaphyS., KarnJ., LoweA.D., SinghM., and SkinnerM.A., EMBO J 9, 4145–4153, 1990.
RoyS., DellingU., ChenC.-H., RosenC.A., and SonenbergN., Genes Dev 4, 1365–1373, 1990.
FranzaB.R.Jr., RauscherJ.J.III, JosephsS.F., and CurranT., Science 239, 1150–1153, 1986.
DinterH., ChiuR., ImagawaM., KarinM., and JonesK.A., EMBO J 6, 4067–4071, 1987.
KaufmanJ.D., ValandraG., RodriguezG., BusharG., GiriC., and NorcrossM., Mol Cell Biol 7, 3759–3766, 1987.
NabelG.J. and BaltimoreD., Nature 326, 711–713, 1987.
JonesK., KadonagaJ., LuciwP., and TjianR., Science 232, 755–759, 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sakuragi, JI., Sakuragi, S., Ueda, S. et al. Functional analysis of simian immunodeficiency virus SIVAGM long terminal repeat. Virus Genes 12, 21–25 (1996). https://doi.org/10.1007/BF00369997
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00369997