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Mass balance considerations for chemical recycling in two phase aqueous fractionation of proteins

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Abstract

Mathematical modelling, using mass balances, of a polymer/salt two phase aqueous protein fractionation process with phase chemical recycle, is used to investigate the effect of several process parameters on phase chemical requirement. Data available on whey protein fractionation to produce α-lactalbumin and β-lactoglobulin are used. Parameters considered include the fraction of phase chemicals recycled, concentrating of phase chemicals in the recycles, bottom phase polymer concentration, concentrating of protein in the feed stream, and the effect of protein build-up due to recycling. The analysis shows that considerable savings in phase chemical requirement can be made by selecting appropriate process parameter values.

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Abbreviations

f s :

stage 1 salt recycle concentration factor

i :

subscript i (= 1 or 2) represents a stage

j :

subscript j (= A, B, or O) represents a protein type (A: protein A, B: protein B, O: contaminant proteins)

K ji :

partition co-efficient of protein j in stage i=protein concentration in top phase divided by that in bottom phase

n p :

fraction of polymer recycled from stage 2 to stage 1

n s :

fraction of stage 1 salt recycled after ultrafiltration

V v :

percentage volume ratio=volume of top phase/total volume

V r :

phase volume ratio=volume of top phase/volume of bottom phase

Z 1,Z 2,Z 3 :

constants

ρ p :

polymer density

ρ s :

salt density

x b :

polymer concentration in bottom phase

x i :

polymer concentration in a stage

x rp :

polymer concentration in polymer recycle entering stage 1

x rs :

polymer concentration in salt recycle entering stage 1

x t :

polymer concentration in top phase

y b :

salt concentration in bottom phase

y i :

salt concentration in a stage

y rp :

salt concentration in polymer recycle entering stage 1

y rs :

salt concentration in salt recycle entering stage 1

y t :

salt concentration in top phase

E 2 :

flowrate of stage 2 recycle bleed/make-up

F :

feed flowrate

G :

top phase flowrate leaving stage 1

H :

top phase flowrate leaving stage 2

I :

bottom phase flowrate leaving stage 1

J :

bottom phase flowrate leaving stage 2

P 1 :

fresh polymer flowrate to stage 1

R 1 :

permeate flowrate leaving ultrafiltration unit after stage 1

R 2 :

permeate flowrate leaving ultrafiltration unit after stage 2

R p :

flowrate in polymer recycle entering stage 1

R s1 :

flowrate in salt recycle entering stage 1

R s2 :

flowrate in salt recycle entering stage 2

S 1 :

fresh salt flowrate to stage 1

S 2 :

fresh salt flowrate to stage 2

C jf :

protein concentration in feed

C jg :

protein concentration in top phase leaving stage 1

C jh :

protein concentration in top phase leaving stage 2

C ji :

protein concentration in bottom phase leaving stage i

C jrp :

protein concentration in polymer recycle entering stage 1

C jsi :

protein concentration in stage i

C nm :

total protein concentration in stage or stream m

C ui :

concentration of total protein and polymer after stage i ultrafiltration

References

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Authors and Affiliations

  1. National Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland

    J. J. Fitzpatrick

  2. Department of Agricultural Engineering, Texas A & M University, 77843, College Station, TX, USA

    C. R. Engler

Authors
  1. J. J. Fitzpatrick
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  2. C. R. Engler
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Fitzpatrick, J.J., Engler, C.R. Mass balance considerations for chemical recycling in two phase aqueous fractionation of proteins. Bioprocess Eng. 13, 149–155 (1995). https://doi.org/10.1007/BF00369698

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  • DOI: https://doi.org/10.1007/BF00369698

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