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Production of xylitol from raw wood hydrolysates by Debaryomyces hansenii NRRL Y-7426

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Abstract

Xylose solutions were obtained from the hemicellulosic fraction of Eucalyptus globulus by means of sulphuric acid treatments performed under selected operational conditions. The hydrolysates were neutralized, supplemented with nutrients, sterilized and utilised as fermentation media for xylitol production using the yeast Debaryomyces hansenii NRRL Y-7426. Preliminary experiments allowed the adaptation of the strain to the culture media. Further fermentation trials were performed with adapted cells according to an incomplete, factorial design of experiments. Empirical models describing the bioconversion were derived from the experimental results. The effects of three operational variables (nutrient concentration, initial pH and shaking speed) on both xylose consumption and xylitol production rates were described by significant mathematical equations. Additional aspects in relation to the process were also discussed.

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  1. Department of Chemical Engineering, University of Vigo (Campus Orense), 32004, Las Lagunas, Orense, Spain

    J. C. Parajó, H. Domínguez & J. M. Domínguez

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  1. J. C. Parajó
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  2. H. Domínguez
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  3. J. M. Domínguez
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The authors are grateful to Xunta de Galicia and “Orember” for their financial support to this work; as well as to Ms. Rocío Rodríguez Fontán for her excellent technical assistance.

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Parajó, J.C., Domínguez, H. & Domínguez, J.M. Production of xylitol from raw wood hydrolysates by Debaryomyces hansenii NRRL Y-7426. Bioprocess Eng. 13, 125–131 (1995). https://doi.org/10.1007/BF00369695

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