Abstract
The introduction of more imaginative enrichment and isolation procedures has permitted the isolation of pure cultures of thermotolerant methylotrophic bacteria, a group that was previously unknown. One potential application for such bacteria is in the aerobic biotreatment of petrochemical industry wastewaters at elevated temperatures. Here, the growth and biooxidation characteristics of one such bacterium, Bacillus sp. NCIB 12522, in co-culture with a Gram-negative thermophilic non-methylotrophic solvent utilizing bacterium, NA 17, on a mixture of methanol, ethanol and isopropanol, under both steady and transient state continuous flow culture conditions are reported. The results indicate that at dilution rates <0.2 h−1 effective biooxidation can be achieved at temperatures between 50° and 57 °C. As a result of step increases in bioreactor feed concentrations, the fraction of the methylotroph present in the co-culture changed according to whether the methanol or the ethanol concentrations were increased, but when isopropanol was increased, no change in the methylotroph fraction occurred between the initial and final steady states.
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References
Wilkinson, T. G.; Topiwala, H. H.; Hamer, G.: Interactions in a mixed population growing on methane in continuous culture. Biotechnol. Bioengng. 16 (1974) 41–59
Linton, J. D.; Buckee, J. C.: Interactions in a methane-utilizing mixed bacterial culture in chemostat culture. J. Gen. Microbiol. 101 (1977) 219–225
Hamer, G.: A biotechnological approach to the treatment of wastewater from petrochemicals manufacture. Instn. Chem. Engrs. Symp. Ser. 77 (1983) 87–101
Wilkinson, T. G.; Hamer, G.: The microbial oxidation of mixtures of methanol, phenol, acetone and isopropanol with reference to effluent purification. J. Chem. Technol. Biotechnol. 29 (1979) 56–67
Bungay, H. R.; Bungay, M. L.: Microbial interactions in continuous culture. Adv. Appl. Microbiol. 10 (1968) 269–290
Fredrickson, A. G.: Behaviour of mixed cultures of microorganisms. Ann. Rev. Microbiol. 31 (1977) 63–87
Harder, W.; Veldkamp, H.: Competition of marine psychrophilic bacteria at low temperatures. Antonie van Leeuwenhoek J. Microbiol. Serol. 37 (1971) 51–63
Veldkamp, H.; van Gemerden, H.; Harder, W.; Lannbroek, H. J.: Competition among bacteria: an overview. In Current Perspectives in Microbial Ecology, eds. M. J. Klug & C. A. Reddy, ASM Washington (1984) 279–290
Harder, W.; Kuenen, J. G.; Matin, A.: A review. Microbial selection in continuous cultures. J. Appl. Bacteriol. 43 (1977) 1–24
Monod, J.: Recherches sur la croissance des cultures bactériennes. Paris: Hermann 1942
Egli, T.; Bosshard, C.; Hamer, G.: Simultaneous utilization of methanol-glucose mixtures by Hansenula polymorpha in chemostat; influence of dilution rate and mixture composition on utilization pattern. Biotechnol. Bioengng. 28 (1986) 1735–1741
Harder, W.; Dijkhuizen, L.: Strategies of mixed substrate utilization in microorganisms. Phil. Trans. Roy. Soc. London B 297 (1982) 459–480
Gottschal, J. C.; de Vries, S.; Kuenen, J. G.: Competition between the facultative chemolithotrophic Thiobacillus A2, an obligately chemolithotrophic Thiobacillus and a heterotrophic Spirillum for inorganic and organic substrates. Arch. Microbiol. 121 (1979) 241–249
Kuenen, J. G.; Robertson, L. A.: Interactions between obligately and facultatively chemolithotrophic sulphur bacteria. In: Dean, A. C. R.; Ellwood, D. C.; Evans, C. G. T. (Eds.): Continuous Culture 8. SCI, London/Ellis Horwood, Chichester (1984) 139–158
Bitzi, U.: Abbau organischer Lösungsmittel mit bakteriellen Misch- und Reinkulturen. Diss. ETH Zürich, No. 8118 (1986) 119 pp
Hamer, G.: Continuous culture kinetics and activated sludge processes. In: Dean, A. C. R.; Ellwood, D. C.; Evans, C. G. T. (Eds.): Continuous Culture 8. SCI, London/Ellis Horwood, Chichester (1984) 169–184
Zlokarnik, M.: Bioengineering aspects of aerobic waste water purification. Ger. Chem. Engng. 6 (1983) 183–197
Al-Awadhi, N.; Egli, T.; Hamer, G.; Wehrli, E.: Thermotolerant and thermophilic solvent-utilizing methylotrophic, aerobic bacteria. System. Appl. Microbiol. 11 (1989) 207–216
Al-Awadhi, N.; Egli, T.; Hamer, G.; Wehrli, E.: Thermophilic solvent-utilizing non-methylotrophic aerobic bacteria. System. Appl. Microbiol. 11 (1989) submitted
Al-Awadhi, N.; Egli, T.; Hamer, G.: Growth characteristics of a thermotolerant methylotrophic Bacillus sp. (NCIB 12522) in batch culture. Appl. Microbiol. Biotechnol. 29 (1988) 485–493
Al-Awadhi, N.: The characterization and physiology of some thermotolerant and thermophilic solvent-utilizing bacteria. Diss. ETH Zürich, No. 8810 (1989) 195 pp
Egli, T.; Weilenmann, H.-U.; El-Banna, T.; Auling, G.: Gramnegative, aerobic nitrilotriacetate-utilizing bacteria from wastewater and soil. System. Appl. Microbiol. 10 (1989) 297–305
Hamer, G.: Lysis and “cryptic”growth in wastewater and sludge treatment processes. Acta Biotechnol. 5 (1985) 117–127
Topiwala, H. H.; Hamer, G.: Effect of wall growth in steady state continuous cultures. Biotechnol. Bioengng. 13 (1971) 919–922
Chi, C. T.; Howell, J. A.: Transient behaviour of a continuous stirred tank biological reactor utilizing phenol as an inhibitory substrate. Biotechnol. Bioengng. 18 (1976) 63–80
Taylor, D. G.; Trudgill, P. W.; Cripps, R. E.; Harris, P. R.: The microbial metabolism of acetone. J. Gen. Microbiol. 118 (1980) 159–170
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Al-Awadhi, N., Hamer, G. & Egli, T. The biooxidation of methanol, ethanol and isopropanol by a defined co-culture at elevated temperatures. Bioprocess Eng. 5, 39–45 (1990). https://doi.org/10.1007/BF00369645
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DOI: https://doi.org/10.1007/BF00369645