Abstract
Two immobilized bacterial cultures with the ability to metabolize 6-amino-2-naphthalenesulfonic acid (6A2NS) and 2-naphthalene-sulfonic acid (2NS) were investigated under suboptimal environmental growth conditions. The cultures were employed in continuously operated airlift loop-reactors. The physico-chemical growth parameters such as pH, temperature and dissolved oxygen concentration were varied. It was found that a decreasing growth rate in suboptimal conditions was compensated by increasing biomass concentration over a wide range. Operated continuously for more than 20 months, the 6A2NS-degrading system appeared to be reliable. After pH-shockloadings and long-term oxygen default, the immobilized microorganisms recovered almost immediately and stable operating conditions were achieved again within less than 48 h. These remarkable results were sustained with the 2NS degrading system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nörtemann, B.; Baumgarten, J.; Rast, H.-G.; Knackmuss, H.-J.: Bacterial communities degrading amino- and hydroxynaphthalene-2-sulfonates. Appl. Environ. Microbiol. Vol. 52 (1986) 1195–1202
Brilon, C.; Beckmann, W.; Hellwig, M.; Knackmuss, H.-J.: Enrichment and isolation of naphthalenesulfonic acid utilizing pseudomonads. Appl. Environ. Microbiol. 42 (1981) 39–43
Brilon, C.; Beckmann, W.; Knackmuss, H.-J.: Catabolism of naphthalenesulfonic acids by pseudomonas sp. A3 and pseudomonas sp. C22. Appl. Environ. Microbiol. 42 (1981) 44–45
Wagner, K.; Hempel, D. C.: Biodegradation by immobilized bacteria in an airlift loop-reactor — influence of biofilm diffusion limitation. Biotechnol. Bioeng. 31 (1988) 559–566
Diekmann, R.; Nörtemann, B.; Hempel, D. C.; Knackmuss, H. J.: Degradation of 6-aminonaphthalene-2-sulphonic acid by mixed cultures —kinetic analysis. Appl. Microbiol. Biotechnol. 29 (1988) p. 85–88
Becker, R.-J.; Hüppe, P.; Wagner, K.; Hempel, D. C.: Einsatz eines Suspensions-Airlift-Schlaufenreaktors zur Reinigung problematischer Abwässer. Chem.-Ing.-Techn. 59 (1987) 6, 486–489
Diekmann, R.; Nörtemann, B.; Hempel, D. C.; Knackmuss, H. J.: Biodegradation of xenobiotics: mineralization of 6-aminonaphthalene-2-sulfonic acid by mixed cultures. Proc. 4th Europ. Congress on Biotechnology (1987), Amsterdam. Extended Abstract Book Vol.1, 85–88, Elsevier Science Publ.
Gerdes-Kühn, M.; Diekmann, R.; Hempel, D. C.: Trägerfixierung von Spezialkulturen — eine wirkungsvolle Methode zum Abbau persistenter Abwasserinhaltsstoffe. Korrespondenz Abwasser, 36 (1989) 776–784
Nörtemann, B.: unpublished data
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Diekmann, R., Naujoks, M., Gerdes-Kühn, M. et al. Effects of suboptimal environmental conditions on immobilized bacteria growing in continuous culture. Bioprocess Eng. 5, 13–17 (1990). https://doi.org/10.1007/BF00369642
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00369642