Skip to main content
SpringerLink
Log in

Adsorption of Bovine Serum Albumin from salt solution onto activated carbon

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

One of the wastewaters from tanning industry (soak liquor) contains 0.4 g/l of dissolved protein. During coagulation and flocculation 41 % of protein was removed. A suggestion has been made to remove the residual protein by adsorption technique. The optimum conditions for adsorption of Bovine Serum Albumin (BSA) on rice bran based activated carbon (RBAC) have been determined. Maximum adsorption of BSA took place at pH 7.O. Ionic strength was found to have influence on the adsorption behaviour. Adsorption capacity of BSA onto charcoal surface decreased with increase in temperature. Enthalpy of adsorption in all cases was found to be within −19 to −57 kJ/mole, indicating exothermic nature of the process. Applicability of adsorption technique to the removal of dissolved protein from soak water has been studied. The maximum removal of protein occurred at pH 7.0 and the ratio of protein removed to weight of adsorbent was 3.22×10−3 g/g.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Krishnamurthy, V.S.; Padmini, K.: Purification of used salt for curing. J. Am. Leather Chemists Assoc. 71 (1982) 36–40

    Google Scholar 

  2. Nandy, S.C.: Some considerations on the utilisation of Bittern salt in curing. Symposium on Raw-Hides and skins during and preservation. (1957) C.L.R.I. Madras, 71–75

    Google Scholar 

  3. Sekaran, G.; Chitra, K.; Shanmugasundaram, K.A.; Madhavakrishna, W.: Recovery of sodium chloride from scraped salt obtained from solar evaporation pans. Leather Science 30 (1983) 191–192

    Google Scholar 

  4. Shegal, P.K.: New process for reclaiming used common salt. Leather Science. 23 (1976) 236–239

    Google Scholar 

  5. Simoncini, A.: A study on the reuse of curing salt (Denaturation by sodium chloride). Symposium on Fuller and better utilisation of hides. C.L.R.I. Madras. (1964) 35–48

    Google Scholar 

  6. Aly, O.M.; Faust, S.D.: Removal of 2,4-dichloro phenoxy acetic acid derivatives from natural wastes. J. Am. Wat. Wks. Assoc., 57 (1965) 221–230

    Google Scholar 

  7. Bailey, G.M.; White, J.L.; Rothleg, T.: Adsorption of organic herbicides by montomorillonite-role of pH and chemical characteristics of adsorbate. Soil. Sci. Soc. Am. Proc., 32 (1968) 222–233

    Google Scholar 

  8. Barthomens, D.; Ha, B.: Adsorption of benzene and cyclohexane on faujasite type zeolites. J. Chem. Soc. Faraday Trans., 69 (1973) 2158–2165

    Google Scholar 

  9. EI-Dib, M.A.; Aly, O.A.: Comparative adsorption of some organics on powdered carbon. First conference on Mechanical power Engineering Cairo University, 1973

  10. Oberoi, C.K.; Sharma, I.P.; Vermoan, K.S.; Goswami, A.K.: Evaluation of kinetic and thermodynamic parameters to determine the effect of detergent polluted water on clays. Clay Research, 5(1) (1986) 39–45

    Google Scholar 

  11. Poots, U.J.P.; McKay, G.; Healy, J.J.: Removal of basic dye from effluent using wood as adsorbent. J. Wat. Poll. Cont. Fed. 50 (1978) 926–935

    Google Scholar 

  12. Stephen, J.; Allen,; McKay, G.; Khader, K.Y.H.: Equilibrium adsorption isotherms for Basic dyes on to Lignite. J. Chem. Tech. Biotechnol. 45 (1989) 291–302

    Google Scholar 

  13. Weber, W.J.; Asce, Jr. J.M.; Morris, J.C.: Kinetics of adsorption on carbon from solutions. Journal of Sanitary Engineering Division, proceedings of the American Society of Civil Engineers 89 (1963) 31–59

    Google Scholar 

  14. Gupta, G.S.; Prasad, G.; Singh, V.N.: Removal of Chrome Dye from aqueous solutions by mixed adsorbents fly ash and Coal. Wat. Res. 24(1) (1990) 45–50

    Google Scholar 

  15. Joyce, R.S.; Subenik, V.A.: Feasibility of granular activated carbon adsorption for wastewater renovation. Pub. Health Ser. Pub. No. 999 May, (1964)

  16. McKay, G.: The adsorption of dye stuffs from solutions with activated carbon. J. Chem. Tech. Biotechnol. 32 (1982) 127–39

    Google Scholar 

  17. Sekaran, G.: Studies on removal of pollutants from tannery effluent. Ph.D. thesis, Madras University, Madras, India (1988)

    Google Scholar 

  18. Standard methods for the examination of water and wastewater 17th Ed. Published jointly with APHA, AWWA, WPCF (1989)

  19. Giles, H.; Smith, D.; Huitson, A.: A general treatment and classification of the solute adsorption isotherm (I). J. Colloid Interface Sci. 47 (1974) 755–765

    Google Scholar 

  20. Giles, C.H.; D'Silva, A.P.; Easton, I.A.: A general treatment and classification of the solute adsorption isotherm (II). J. Colloid Interface Sci. 47 (1974) 766–778

    Google Scholar 

  21. Nassar, M.M.; El-Geundi.: Comparative cost of colour removal from textile effluents using natural adsorbents. J. Chem. Tech. Biotechnol. 50 (1991) 257–264

    Google Scholar 

  22. Singh, B.K.; Rawat, N.S.: Comparative sorption kinetics studies of phenolic compounds on fly ash and impregnated fly ash. J. Chem. Tech. Biotechnol. 61 (1994) 57–65

    Google Scholar 

  23. Ghosh, B.N.; Chattoraj, D.K.: Stability and electrokinetic potential of Al2O3 hydrosol. J. Indian Chem. Soc. 35 (1958) 315–319

    Google Scholar 

  24. Norde, W.; Lyklema, J.: The adsorption of human plasma albumin and Bovine Pancreas Ribonuclease at negatively charged polystyrene surfaces. J. Colloid and Interface Sci. 66 (1978) 257–302

    Google Scholar 

  25. Armstrong, D.E.; Chesters, G.: Properties of protein-bentonite complexes as influenced by equilibrated conditions. Soil. Sci. 98 (1964) 39–52

    Google Scholar 

  26. Morrissey, B.W.; Stormbeg, R.R.: The conformation of adsorbed blood proteins by infra-red bound fraction measurements. J. Colloid and Interface Science. 46 (1974) 152–164

    Google Scholar 

  27. Macritchie, F.: Adsorption of proteins at the solid/liquid interface. J. Colloid and Interface Sci. 38 (1972) 484–488

    Google Scholar 

  28. Chattoraj, D.K.; Bull, H.B.: Electrophoresis of adsorbed protein. J. Am. Chem. Soc. 81 (1959) 5128–5133

    Google Scholar 

  29. McLaren, A.D.: Adsorption of proteins at the solid/liquid interface. J. Colloid Interface Sci. 38 484–88

  30. Kobamato, N.; Fofroth, G.; Camp, P.; Amburg, G.V.; Augenstain, L.: Specificity of trypsin adsorption on cellulose, glass and quartz. Biochem. Biophys. Res. Communication 24 (1966) 1622–1627

    Google Scholar 

  31. Pearce, E.I.F.; Bibby, B.G.: Protein adsorption on dental enamel. Arch. oral Biol. 11 (1966) 329–336

    Google Scholar 

  32. McKay, G.; Ramprasad, G.; Mowali, P.P.: Equilibrium studies for the adsorption of dye stuffs from aqueous solutions by Low cost Methods. Water, Air and Soil pollution. 29 (1986) 273–283

    Google Scholar 

  33. Panday, K.K; Prasad, G.; Singh, V.N.: Adsorption technique for removal of oxalic acid. Nat. Acad. Sci. Lett. 6 (1984) 415–417

    Google Scholar 

  34. Gupta, G.S.; Prasad, G.; Singh, V.N.: Removal of colour from wastewater by sorption for reuse. J. Environ. Sci. 111, A23 (1988) 205–217

    Google Scholar 

  35. Tewari, P.H.; Cambell, A.B.; Lee, W.: Adsorption of Co2+ by oxides from aqueous solution. Canad. J. Chem. 50 (1972) 1642–1648

    Google Scholar 

  36. Mitra, S.P.; Chattoraj, D.K.: Some thermodynamic aspects of expanded and condensed films of BSA adsorbed at the Alumina-Water interface. Ind. J. Biochem. and Biophys. 15 (1978) 147–152

    Google Scholar 

  37. The Hindu, 4th June (1984)

Download references

Author information

Authors and Affiliations

  1. Department of Environmental Technology, Central Leather Research Institute, 600 020, Adyar, Madras, India

    G. Sekaran, M. Mariappan & K. V. Raghavan

Authors
  1. G. Sekaran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Mariappan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. V. Raghavan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sekaran, G., Mariappan, M. & Raghavan, K.V. Adsorption of Bovine Serum Albumin from salt solution onto activated carbon. Bioprocess Engineering 15, 165–169 (1996). https://doi.org/10.1007/BF00369621

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00369621

Keywords

Search

Navigation