Abstract
To facilitate lipase (E.C.3.1.1.3) catalyzed hydrolysis reaction of triglycerides in heterogeneous reaction systems, lipase-surfactant complex (LSC) was prepared by mixing an aqueous solution of lipase and surfactant in ethanol. The LSC prepared from lipase MF-30 derived from Pseudomonas sp. and Nonion SP-60R (sorbitan mono-stearate) showed a high LSC yield and activity recovery. About 30% of the protein in the crude lipase was recovered in the LSC precipitate, and further addition of surfactant to the supernatant was ineffective. Our preparation method for the selective separation of the catalytic active protein from a crude enzyme was suggested.
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The authors are grateful to Amano Pharmaceutical Co., Ltd. for providing crude lipases, to Dai-ichi Kogyo Seiyaku Co., Ltd. and NOF Corporation for supplying surfactants. We also acknowledge Dr. J.B. Snape for his helpful advice in the preparation of the manuscript. This work was partly supported by Bio-Renaissance of the Ministry of Agriculture, Forestry and Fisheries (BRP 94-I-A).
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Isono, Y., Nabetani, H. & Nakajima, M. Preparation of lipase-surfactant complex for the catalysis of triglyceride hydrolysis in heterogeneous reaction systems. Bioprocess Engineering 15, 133–137 (1996). https://doi.org/10.1007/BF00369616
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DOI: https://doi.org/10.1007/BF00369616