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Modelling of continuous acetonobutylic fermentation

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Abstract

A model of continuous acetonobutylic fermentation is proposed. This model correctly portrays the predominantly solvents formation observed at acidic extracellular pH and predominantly acids production at more neutral pH, as well as observed effects of dilution rate and feed substrate on products' concentrations. A fair agreement between experimental and theoretical predictions is achieved for a broad range of operating variables.

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Abbreviations

A gl−1 :

Acetone concentration

AA gl−1 :

Acetic acid concentration

B gl−1 :

Butanol concentration

BA gl−1 :

Butyric acid concentration

D h−1 :

Dilution rate

DBA gl−1 :

Butyrate concentration

E gl−1 :

Ethanol concentration

r A gl−1 h−1 :

Rate of acetone formation

r AA gl−1 h−1 :

Rate of acetic acid accumulation

r B gl−1 h−1 :

Rate of butanol formation

r BA gl−1 h−1 :

Rate of butyric acid accumulation

r E gl−1 h−1 :

Rate of ethanol formation

r S gl−1 h−1 :

Rate of substrate consumption

r X gl−1 h−1 :

Rate of biomass growth

S gl−1 :

Substrate concentration

UBA gl−1 :

Butyric acid concentration (undissociated form)

Y x/s g/g:

Theoretical yield of cells based on substrate consumed

Y p/s g/g:

Theoretical yield of product based on substrate consumed

X gl−1 :

Biomass concentration

μ h−1 :

Specific growth rate

c :

refers to rate of conversion

i :

intracellular

o :

extracellular

p :

refers to formation

References

  1. Krouwel, P. G.; Groot, W. J.; Kossen, N. W. F.: Continuous IBE fermentation by immobilized growing Clostridium beijerinckii cells in a stirred-tank fermentor. Biotechnol. Bioeng. 25 (1983) 281–299

    Google Scholar 

  2. Leung, J. Y. C.; Wang, D. I. C.: Production of acetone and butanol by Clostridium acetobutylicum in continuous culture using free and immobilized cells. Proc. 2nd World Chem. Eng. Conf. 1 (1984) 348–355

    Google Scholar 

  3. Mulchandani, A.; Volesky, B.: Modelling of the acetone-butanol fermantation with cell retention. Canad. J. Chem. Eng. 64 (1986) 625–631

    Google Scholar 

  4. Bahl, H.; Andersch, W; Braun, K.; Gottschalk, G.: Effect of pH and butyrate concentration on the production of acetone and butanol by Clostridium acetobutylicum grown in continuous culture. Eur. J. Appl. Microbiol. Biotechnol. 14 (1982) 17–20

    Google Scholar 

  5. Monot, F.; Engasser, J. M.; Petitdemange, H.: Influence of pH and undissociated butyric acid on the production of acetone and butanol in batch cultures of Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 19 (1984) 422–426

    Google Scholar 

  6. Roos, J. W.; McLaughlin, J. K.; Papoutsakis, E. T.: The effect of pH on nitrogen supply, cell lysis, and solvent production in fermentations of Clostridium acetobutylicum. Biotechnol. Bioeng. 27 (1985) 681–694

    Google Scholar 

  7. Gottwald, M.; Gottschalk, G.: The internal pH of Clostridium acetobutylicum and its effects on the shift from acid to solvent formation. Arch. Microb. 143 (1985) 42–46

    Google Scholar 

  8. Huang, L.; Forsberg, C. W.; Gibbins, L. N.: Influence of external pH and fermentation products on Clostridium acetobutylicum intracellular pH and cellular distribution of fermentation products. Appl. Environ. Microbiol. 51 (1986) 1230–1234

    Google Scholar 

  9. Terraciano, J. S.; Kashket, E. R.: Intracellular conditions required for initiation of solvent production by Clostridium acetobutylicum. Appl. Environ. Microbiol. 52 (1986) 86–91

    Google Scholar 

  10. Hüsemann, M. H. W; Papoutsakis, E. R.: Solventogenesis in Clostridium acetobutylicum fermentation related to carboxylic acid and proton concentrations. Biotechnol. Bioeng. 32 (1988) 843–852

    Google Scholar 

  11. Soni, B. K.; Soucaille, P.; Goma, G.: Effect of phosphate cycling on production of acetone-butanol by Clostridium acetobutylicum in chemostat culture. Biotechnol. Bioeng. Symp. 17 (1986) 239–252

    Google Scholar 

  12. Huang, L.; Gibbins, L. N.; Forsberg, C. W: Transmembrane pH gradient and membrane potential in Clostridium acetobutylicum during growth under acetogenic and solventogenic condiions. Appl. Environ. Microbiol. 50 (1985) 1043–1047

    Google Scholar 

  13. Kell, D. B.; Peck, M. W.; Rodger, G.; Morris, J. G.: On the permeability to weak acids and bases of the cytoplasmic membrane of Clostridium pasteurianum. Biochem, Biophys. Res. Commun. 99 (1981) 81–88

    Google Scholar 

  14. Herrero, A. A.; Gomez, R. F.; Snedecor, B.; Tolman, C. J.; Roberts, M. F.: Growth inhibition of Clostridium thermocellum by carboxylic acids: a mechanism based on uncoupling by weak acids. Appl. Microbiol. Biotechnol. 22 (1985) 53–62

    Google Scholar 

  15. Izard, A.; Goma, G.; Soucaille, P.: Effects of various alcoholic supplements on the growth rate of Clostridium acetobutylicum ATCC 824. Appl. Microbiol. Biotechnol. 31 (1989) 179–183

    Google Scholar 

  16. Hüsemann, M. H. W.; Papoutsakis, E. T.: Effects of propionate and acetate additions on solvent production in batch cultures of Clostridium acetobutylicum. Appl. Environ. Microbiol. 56 (1990) 1497–1500

    Google Scholar 

  17. Soucaille, P.; Goma, G.: Acetonobutylic fermentation by Clostridium acetobutylicum ATCC 824: Autobacteriocin production, properties, and effects. Curr. Microbiol. 13 (1986) 163–169

    Google Scholar 

  18. Ferras, E.; Minier, M.; Goma, G.: Acetonobutylic fermentation: Improvement of performances by coupling continuous fermentation and Ultrafiltration. Biotechnol. Bioeng. 28 (1986) 523–533

    Google Scholar 

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Jarzębski, A.B., Goma, G. & Soucaille, P. Modelling of continuous acetonobutylic fermentation. Bioprocess Engineering 7, 357–361 (1992). https://doi.org/10.1007/BF00369491

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