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A kinetic model and simulation of starch saccharification and simultaneous ethanol fermentation by amyloglucosidase and Zymomonas mobilis

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Abstract

A mathematical model is described for the simultaneous saccharification and ethanol fermentation (SSF) of sago starch using amyloglucosidase (AMG) and Zymomonas mobilis. By introducing the degree of polymerization (DP) of oligosaccharides produced from sago starch treated with α-amylase, a series of Michaelis-Menten equations were obtained. After determining kinetic parameters from the results of simple experiments carried out at various substrate and enzyme concentrations and from the subsite mapping theory, this model was adapted to simulate the SSF process. The results of simulation for SSF are in good agreement with experimental results.

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Abbreviations

α g/g:

rate coefficient of production

μ max 1/h:

maximum specific growth rate

E %, v/w:

AMG concentration

G 1 mmol/l:

glucose concentration

G c mmol/l:

glucose concentration consumed

G f mmol/l:

glucose concentration formed

G n mmol/l:

n-mer maltooligosaccharide concentration

K i g/l:

ethanol inhibition constant for ethanol production

K g mmol/l:

glucose inhibition constant for glucose production

K p mmol/l:

glucose limitation constant for ethanol production

K x mmol/l:

glucose limitation constant for cell growth

K m,n mmol/l:

Michaelis-Menten constant for n-mer oligosaccharide

k e %, v/w:

enzyme limitation constant

k es :

proportional constant

k max, n 1/s:

maximal velocity for n-mer digestion

k s g/l:

substrate limitation constant

m s g/g:

maintenance energy

MW n g/mol:

molecular weight of n-mer oligosaccharide

P g/l:

ethanol concentration

P 0 g/l:

initial ethanol concentration

P m g/l:

maximal ethanol concentration

Q pm g/(g · h):

maximum specific ethanol production rate

S n mmol/h:

branched n-mer oligosaccharide concentration

S 0 g/l:

initial starch concentration

S sta g/l:

starch concentration

S tot g/l:

total sugar concentration

V max, n 1/h:

maximum digestion rate of n-mer oligosaccharide

V 0 g/(l · h):

initial glucose formation rate

X g/l:

cell mass

X 0 g/l:

initial cell mass

Y p/s g/g:

ethanol yield

Y x/s g/g:

cell mass yield

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Michigan, 48109-2136, Ann Arbor, Michigan, USA

    C. -G. Lee

  2. Laboratory of Metabolic Engineering, Genetic Engineering Research Institute Korea Institute of Science and Technology, P.O. Box 17, 305-606, Taedok Science Town, Taejon, Korea

    C. H. Kim & S. K. Rhee

Authors
  1. C. -G. Lee
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  2. C. H. Kim
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  3. S. K. Rhee
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Lee, C.G., Kim, C.H. & Rhee, S.K. A kinetic model and simulation of starch saccharification and simultaneous ethanol fermentation by amyloglucosidase and Zymomonas mobilis. Bioprocess Engineering 7, 335–341 (1992). https://doi.org/10.1007/BF00369488

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