Abstract
Production of t-PA by human embryonic lung fibroblasts, IMR-90 cells, is regulated by negative feedback control. The increase in the concentration of the extracellular t-PA lead to a reduction of the production. Therefore, we investigated the application of t-PA adsorption column to ceramic bed reactor to promote t-PA production. Amberlite XAD-8 was selected out as an adsorbent, because it is autoclavable and can adsorb 32,000 IU of t-PA per g wet gel. The t-PA adsorption column was located in the medium recirculation line to the vessel. On the other hand, medium was recirculated between the ceramic bed reactor and the vessel using another flow line. The bioreactor system with the adsorption column was about 2.5 times higher with the resulting cumulative t-PA than that without the adsorption column.
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Mitsuda, S., Matsuda, Y., Kobayashi, N. et al. Tissue plasminogen activator (t-PA) production by human fibroblasts using a bioreactor with t-PA adsorption column. Bioprocess Engineering 7, 137–140 (1991). https://doi.org/10.1007/BF00369425
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DOI: https://doi.org/10.1007/BF00369425