Abstract
The crude enzyme powder from Rhizopus nigricans was immobilized by sorption and subsequent cross-linking with glutaraldehyde on collagen and polytetrafluoroethylene (PTFE) membranes. Lipolytic membranes were applied to plant oils hydrolysis. The comparison of the two types of membranes by calculating the time required to obtain 1 mole of free fatty acids (FFA) from 1 m2 of membrane area, indicates that hydrophobic PTFE membrane is a better one in spite of the fact that the amount of protein sorbed on PTFE membrane is about three times smaller than that for collagen membrane. The hydrolysis of sunflower oil was the most efficient at the temperature of 37 °C and a pH of 7. At these conditions the specific activity after immobilization was about four times higher than that of the soluble enzyme.
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Rucka, M., Turkiewicz, B., Żuk, J.S. et al. Hydrolysis of plant oils by means of lipase from Rhizopus nigricans. Bioprocess Engineering 7, 133–135 (1991). https://doi.org/10.1007/BF00369424
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DOI: https://doi.org/10.1007/BF00369424