Abstract
Fed-batch cultivations of hybridoma cell cultures for antibody production were computer simulated by a detailed structured model, that divides the cell mass into four metabolic compartments and makes use of descriptions of the most important metabolic pathways. Surprisingly the simulations indicate, that online or frequent off-line control of fed-batch hybridoma cultures may lead to low and variable yields of antibody product. With less frequently controlled fed-batch cultures the system is more stable; and there is an optimal time interval for control, that depends on the feeds selected. The product yields in optimal fed-batch cultivations, however, are seldom higher than in comparable batch cultivations.
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Hansen, H.A., Madsen, N.M. & Emborg, C. An evaluation of fed-batch cultivation methods for mammalian cells based on model simulations. Bioprocess Engineering 9, 205–213 (1993). https://doi.org/10.1007/BF00369404
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DOI: https://doi.org/10.1007/BF00369404