Abstract
Five overlapping lambda EMBL-clones, containing the complete bovine lactoferrin gene (LTF), have been used to map this gene by fluorescence in situ hybridization to bovine Chromosome (Chr) band 22q24. Primers derived from promoter and exon I sequences were applied in polymerase chain reactions (PCRs) to DNA samples of a previously characterized panel of somatic cell hybrid lines, allowing the assignment of the bovine lactoferrin locus to syntenic group U12. These results permit the assignment of syntenic group U12 to bovine Chr 22.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ansari, H.A., Pearce, P.D., Maher, D.W. (1993). Regional assignment of anchored reference loci to sheep chromosomes. 8th North American Colloquium on Domestic Animal Cytogenetics and Gene Mapping, A. King, ed., July 13–16.
Baranov, V.S., Schwartzman, A.L., Gorbunova, V.N., Gaitskhoki, V.S., Rubtsov, N.B., Timchenko, N.A., Neifakh, S.A. (1987). Chromosomal localization of ceruoplasmin and transferrin genes in laboratory rats, mice and in man by hybridization with specific DNA probes. Chromosoma 96, 60–66.
Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teal, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994). A genetic linkage map of the bovine genome. Nature Genet. 6, 227–235.
Bishop, M.D., Kappes, S.A., Keele, J.W., Stone, R.T., Sunden, S.L.F., Hawkins, G.A., Solinas Toldo, S., Fries, R., Grosz, M.D., Yoo, J., Cundiff, L.W., Beattie, C.W. (1994). A genetic linkage map for cattle. Genetics 136, 619–639.
Bowman, B.H., Yang, F., Adrian, G.S. (1988). Transferrin: evolution and genetic regulation of expression. Adv. Genet. 25, 1–38.
Carritt, B., Naylor, S.L. (1993). Report of the committee on the genetic constitution of chromosome 3. In Genome Priority Reports, Chromosome Coordinating Meeting 1992 (CCM 92), A.J. Cuticchia, P.L. Pearson, H.P. Klinger, eds. (Baltimore, Md.: Johns Hopkins University) pp. 184–220.
Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cytogenet. Cell Genet. 43, 132–139.
Cramer, E., Pryzwanski, K.B., Villeval, J.I., Testa, U., Breton-Gorious, J. (1985). Ultrastructural localization of lactoferrin and myeloperoxidase in human neutrophiles by immunogold. Blood 65, 423–432.
Fries, R., Eggen, A., Womack, J.E. (1993). The bovine genome map. Mammalian Genome 4, 405–428.
Goodman, R.E., Schanbacher, F.L. (1991). Bovine lactoferrin mRNA: sequence, analysis and expression in the mammary gland. Biochem. Biophys. Res. Commun. 180, 75–84.
Guérin, G., Eggen, A., Vaiman, D., Nocart, M., Laurent, P., Béchet, D., Ferrara, M. (1994). Further characterization of a somatic cell hybrid panel: ten new assignments to the bovine genome. Anim. Genet. 25, 31–35.
Hediger, R., Ansari, H.A., Stranzinger, G.F. (1991). Chromosome banding and gene localizations support extensive conservation of chromosome structure between cattle and sheep. Cytogenet. Cell Genet. 57, 127–134.
ISCNDA (1989). International System for Cytogenetic Nomenclature of Domestic Animals. D. DiBerardino, H. Hayes, R. Fries, S. Long, eds. Cytogenet. Cell Genet. 53, 65–79.
LeBeau, M.M., Diaz, M.O., Yang, F., Plowman, G.D., Brown, J.P., Rowley, J.D. (1985). Molecular analysis of transferrin (TF), transferrin receptor (TFR) and p97 antigen genes in the INV(3) and T(3;3) in acute nonlymphocytic leukemia (ANLL). Am. J. Hum. Genet. 37, 4.
Le Provost, F., Nocart, M., Guérin, G., Martin, P. (1994). Lactoferrin: goat cDNA characterization and assignment of the relevant locus to bovine U12 synteny group. submitted.
Lichter, P., Tang, C.-J.C., Call, K., Hermanson, G., Evans, G.A., Housman, D., Ward, D.C. (1990). High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247, 64–69.
Masson, P.L., Heremann, J.F. (1971). Lactoferrin in milk from different species. Comp. Biochem. Physiol. B, 39, 119–129.
McCombs, J.L., Teng, C.T., Pentecost, B.T., Magnuson, V.L., Moore, C.M., McGill, J.R. (1988). Chromosomal localization of human lactotransferrin gene (LTF) by in situ hybridization. Cytogenet. Cell Genet. 47, 16–17.
Mead, P.E., Tweedie, J.W. (1990). c-DNA and protein sequence of bovine lactoferrin. Nucleic Acids Res. 18, 7167.
O'Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.
Pierce, A., Colavizza, D., Benaissa, M., Maes, P., Tartar, A., Montreuil, J. (1991). Molecular cloning and sequence analysis of bovine lactotransferrin. Eur. J. Biochem. 196, 177–184.
Rabin, M., McClelland, A., Kuhn, L., Ruddle, F.H. (1985). Regional localization of the human transferrin receptor gene to 3q26.2-qter. Am. J. Hum. Genet. 37, 1112–1116.
Sanchez, L., Calvo, M., Brock, J.H. (1992). Biological role of lactoferrin. Arch. Dis. Child. 67, 657–661.
Sawatzki, G. (1987). The role of iron binding proteins in bacterial infections. In Iron Transport in Microbes, Plants and Animals. Winkelmann, A., vonder Helm, P., and Neilands, U., eds. (Winheim: VCH), pp. 477–498.
Seabright, M. (1971). A rapid banding technique for human chromosomes. Lancet 2, 971.
Seyfert, H.M., Kühn, C. (1994). Characterization of a first bovine lactoferrin gene variant, based on an EcoRI polymorphism. Anim. Genet. 25, 54.
Seyfert, H.M., Tuckoricz, A., Interthal, H., Koczan, D., Hobom, G. (1994). Structure of the bovine lactoferrin-encoding gene and its promoter. Gene 143, 265–269.
Solinas Toldo, S., Fries, R., Steffen, P., Neibergs, H.L., Barendse, W., Womack, J.E., Hetzel, D.J.S., Stranzinger, G. (1993). Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes. Mamm. Genome 4, 720–727.
Teng, C.T., Pentecost, B.T., Marshall, A., Solomon, A., Bowman, B.H., Lalley, P.A., Naylor, S.L. (1987). Assignment of the lactotransferrin gene to human chromosome 3 and to mouse chromosome 9. Somat. Cell Mol. Genet. 13, 689–693.
Threadgill, D.S., Kraus, J.P., Krawetz, S.A., Womack, J.E. (1991). Evidence for the evolutionary origin of human chromosome 21 from comparative gene mapping in the cow and mouse. Proc. Natl. Acad. Sci. USA 88, 154–158.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schwerin, M., Toldo, S.S., Eggen, A. et al. The bovine lactoferrin gene (LTF) maps to Chromosome 22 and syntenic group U12. Mammalian Genome 5, 486–489 (1994). https://doi.org/10.1007/BF00369317
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00369317