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Integrated biological process for olive mill wastewater treatment

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Abstract

The biological process for OMW treatment is based on an aerobic detoxification step followed by methanization step and aerobic post-treatment.

The first aerobic detoxification step of OMW supplemented with sulfate and ammonium was carried out by the growth of Aspergillus niger in a bubble column. This step decreased OMW toxicity and increased its biodegradability because of phenolic compounds degradation. Growth of A. niger resulted in 58% COD removal, with production of biomass containing 30% proteins (w/w). Filtration of OMW was enhanced by this fermentation because the suspended solids were trapped in the mycelium. The filtrate liquid was then methanized using an anaerobic filter packed with flocoor. This reactor showed a short start up and a good stability. COD removal was around 60% and the methane yield (1 CH4/g COD removed) was close to the theoretical yield.

The anaerobic filter effluent was treated in an activated sludge fluidized reactor containing olive husk as a packing material. Husks were maintained in fluidization state by the aeration. This step induces COD removal at 45% and sludge (up to 2 g/dm3).

The entire process allowed a global COD reduction up to 90%; however, the black colour due to polyphenolic compounds with high molecular weight persisted.

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References

  1. Fiestas Ros de Ursinos, J. A.: Différentes utilisations des margines. In Proc. of Séminaire international sur la valorisation des sous produits de l'olivier. FAO-PNUD. Tunisie. Décembre, pp 93–110, 1981

    Google Scholar 

  2. Boari, G.; Brunetti, A.; Passino, R.; Rozzi, A.: Anaerobic digestion of olive oil mill waste-waters. Agricultural Wastes. 10 (1984) 161–175

    Article  Google Scholar 

  3. Balice, V.; Boari, G.; Cera, O.; Abbaticchio, P.: Indagine analitica sulle acque di vegetazione. Nota l. Inquinamento, no. 7–8 (1982) 49–53

  4. Hamdi, M.: The effect of pretreatment and agitation on the batch anaerobic digestion of olive mill wastewaters. Bioresource Technol. 36 (1991) 173–178

    Article  Google Scholar 

  5. Hamdi, M.; Kadir, A.; Garcia, J. L.: The use of Aspergillus niger for bioconversion of olive mill wastewaters. Appl. Microbiol. Biotechnol. 34 (1991) 829–831

    Google Scholar 

  6. Hamdi, M.: A new conception of biological process for olive mill wastewaters treatment. Ph.D. Thesis, (Université de Provence, France) 1991

    Google Scholar 

  7. Ikeda, Y.; Takahashi, E.; Yokogama, K.; Yoshimora, Y: Screening for microorganisms producing gallic acid from chineese and tara tannins. J. Ferment. Technol. 50 (1972) 361–370

    Article  Google Scholar 

  8. Cichelli, A.; Solinas, M.: I compostifenolici delle olive e dell'oloi di oliva. Riv. Merceol. 23 (1984) 55–69

    Google Scholar 

  9. Balice, V.; Cera, O.: Acidic phenolic fraction of the juice of olives determined by gas Chromatographic method. Grasas y Aceites 25 (1984) 178–180

    Google Scholar 

  10. Vazequez, R. A.; Maestro, D. R.; Graciani, C. E.: Compentes fenolicos de la aceintuna. II poliphenoles del alpechin. Grasas y Aceites 25 (1974) 341–345

    Google Scholar 

  11. Tanchev, S.; Joncheva, N.; Genov, N.; Codounis, M.: Identification of anthocyanins contained in olives. Georgike Ereuna 4 (1) (1980) 5–13

    Google Scholar 

  12. Kieslich, K.: Microbiol transformations of non-steroid cyclic compounds. John Wiley and Sons, Georg Thieme publishers 1976

  13. Makasinova, S. Y.; Martakov, A. A.: Assimilation of phenolic compounds by fungi of the genus Aspergillus. Tr In-Ta Mikrobiol Ivirusol AN Kazssr 27 (1982) 30–36

    Google Scholar 

  14. Blom, H.; Thomassen, M. S.: Kinetic studies on strawberry anthocyanin hydrolysis by a thermostable anthocyanin-β-glucosidase from Aspergillus niger. Food Chem. 17 (1985) 157–168

    Google Scholar 

  15. Hamdi, M.; Garcia, J. L.: Comparison between anaerobic filter and anaerobic contact process for fermented olive mill wastewaters. Biores. Technol. 38 (1991) 23–29

    Google Scholar 

  16. Knechtel, R. J.: A more economical method for the determination of chemical oxygen demand. Water Pollut. Control May/June (1978) 25–29

  17. Fernandez, M. J.: Olives. In: Rehm, H. J.; Reed, G. (Eds.) Biotechnology, vol. 5. pp 379–397, Verlag Chemie, Weinheim (1983)

    Google Scholar 

  18. Hamdi, M.; BouHamed, H.; Ellouz, R.: Optimization of Aspergillus niger growth on olive mill wastewaters. Appl. Microbiol. Biotechnol. 36 (1991) 285–288

    Google Scholar 

  19. Smith, E. I.; Greenshields, R. N.: Tower fermentation systems and their applications to aerobic process. Chemical Engineer. (1974) 28–34

  20. Hamdi, M.; Ellouz, R.: Use of Aspergillus niger to improve filtration of olive mill wastewaters. J. Chem. Technol. and Biotechnol. (In press 1992)

  21. Field, J. A.; Lettinga, G.: Treatment and detoxification of aqueous spruce bark extracts by Aspergillus niger. Wat. Sci. Tech. 24 (1991) 127–137

    Google Scholar 

  22. Hamdi, M.; Festino, C.; Aubart, C.: Anaerobic digestion of olive mill wastewaters in fully mixed reactors and in fixed film reactors. Process Biochem. (In press 1991)

  23. Rozzi, A.; Passino, R.; Limoni, M.: Anaerobic treatment of olive mill effluents in polyurethane foam bed reactors. Process Biochem. April (1989) 68–74

  24. Nefzaoui, A.; Molina, E.; Outmani, A.; Vanbelle, M.: Ensilados de orujo de aceituna tratados con alcalis. Composicion quimica, digestibilidad in sacco y degradabilidae. Archivos de Zootecnia. 33 (1985) 219–236

    Google Scholar 

  25. Switzenbaum, M. S.: A comparison of the anaerobic filter and the anaerobic expanded/fluidized bed processes. Wat. Sci. Tech. 15 (1983) 345–358

    Google Scholar 

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Hamdi, M., Garcia, J.L. & Ellouz, R. Integrated biological process for olive mill wastewater treatment. Bioprocess Eng. 8, 79–84 (1992). https://doi.org/10.1007/BF00369268

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