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Lack of In vitro evidence for storage of 1,25-dihydroxycholecalciferol (1,25(OH)2D3) and 1,25(OH)2D3 binding protein in skeletal matrix

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Abstract

A few studies have reported on the measurement of 1,25-dihydroxycholecalciferol (1,25(OH)2D3) in bone, using chloroform/methanol extraction and radioreceptor assay. As the significance of bone 1,25(OH)2D3 content was not defined in any of these reports, the objective of the current investigation was to determine whether 1,25(OH)2D3 may be stored in skeletal matrix. Bone powder samples from the iliac crest were extracted in ethylacetate/cyclohexane and 1,25(OH)2D3 isolated from the extract by means of Sephadex LH-20 and high pressure liquid chromatographic separation and subsequently measured by radioimmunoassay (RIA). Within the detection range of the RIA, no 1,25(OH)2D3 could be measured, suggesting that 1,25(OH)2D3 is not stored in skeletal matrix. Vitamin D bone concentrations previously measured may therefore have reflected plasma contamination. Consistent with this hypothesis, only traces of skeletal 1,25(OH)2D3 binding protein were measured when compared with serum values. Although 1,25(OH)2D3 may act as a potential local determinant of bone remodeling, there is no evidence supporting a delayed paracrine function by matrix-derived 1,25(OH)2D3.

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References

  1. Raisz L, Trummel C, Holick M, DeLuca H (1971) Dihydroxycholecalciferol: a potent stimulator of bone resorption in tissue culture. Science 175:768–769

    Google Scholar 

  2. Seino Y, Ishizuka S, Shima M, Tanaka H (1993) Vitamin D in bone formation. Osteoporosis Int 3 (suppl 1):S196-S198

    Google Scholar 

  3. Kurose H, Yamaoke K, Okada S, Nakajima S, Seino Y (1990) Dihydroxyvitamin D3 (1,25(OH)2D3) increases insulin-like growth factor I (IGF-I) receptors in clonal osteoblastic cells. Study on interaction of IGF-I and 1,25(OH)2D3. Endocrinology 126:2088–2094

    Google Scholar 

  4. Petkovich P, Wrana J, Grigoriadis A, Heersche J, Sodek J (1987) Dihydroxyvitamin D3 increases epidermal growth factor receptors and transforming growth factor β-like activity in a bone-derived cell line. J Biol Chem 262:13424–13428

    Google Scholar 

  5. Lips P, Obrant K (1991) The pathogensis and treatment of hip fractures. Osteop Int 1:218–231

    Google Scholar 

  6. Weinstein R, Underwood J, Hutson M, DeLuca H (1984) Bone histomorphometry in vitamin D-deficient rats infused with calcium and phosphorus. Am J Physiol 1:E499-E505

    Google Scholar 

  7. Davies M, Mawer E, Hann J, Taylor J (1986) Seasonal changes in the biochemical indices of vitamin D deficiency in the elderly: a comparison of people in residential homes, longstay wards and attending a day hospital. Age Ageing 15:77–83

    Google Scholar 

  8. Bouillon R, Auwerx J, Lissens W, Pelemans W (1987) Vitamin D status in the elderly: seasonal substrate deficiency causes 1,25-dihydroxycholecalciferol deficiency. Am J Clin Nutr 45:755–763

    Google Scholar 

  9. Lips P, Van Ginkel F, Jongen M, Rubertus F, Van Der Vijgh W, Netelenbos J (1987) Determinants of vitamin D status in patients with hip fracture and in elderly control subjects. Am J Clin Nutr 46:1005–1010

    Google Scholar 

  10. Cooper C, McLaren M, Wood P, Coulton L, Kanis J (1989) Indices of calcium metabolism in women with hip fractures. Bone Miner 5:193–200

    Google Scholar 

  11. Sorensen OH, Lumholtz B, Lund B, Hjelmstrand IL, Mosekilde L, Melsen F, Bishop JE, Norman AW (1982) Acute effects of parathyroid hormone on vitamin D metabolism in patients with the bone loss of aging. J Clin Endocrinol Metab 54:1258–1261

    Google Scholar 

  12. Slovik DM, Adams JS, Neer RM, Holick MF, Potts JT Jr (1981) Deficient production of 1,25-dihydroxy vitamin D in elderly osteoporotic patients. N Engl J Med 305:372–374

    Google Scholar 

  13. Tsai KS, Heath H, Kumar R, Riggs BL (1984) Impaired vitamin D metabolism with aging in women. Possible role in pathogenesis of senile osteoporsis. J Clin Invest 73:1668–1672

    Google Scholar 

  14. Alkalay D, Shany S, Dekel S, (1989) Serum and bone vitamin D metabolites in elective patients and patients after fracture. J Bone Joint Surg [Br] 71:85–87

    Google Scholar 

  15. Sagiv P, Lidor C, Hallel T, Edelstein S (1992) Decrease in bone level of 1,25-dihydroxyvitamin D in women over 45 years old. Calcif Tissue Int 51:24–26

    Google Scholar 

  16. Dequeker J, Mohan S, Finkelman R, Aerssens J, Baylink D (1993) Generalized osteoarthritis associated with increased insulin-like growth factor types I and II and transforming growth factor β in cortical bone from the iliac crest. Arthritis Rheum 36:1702–1708

    Google Scholar 

  17. Bouillon R, De Moor P, Baggiolini EG, Uskokovic MR (1980) A radioimmunoassay for 1,25-dihydroxycholecalciferol. Clin Chem 26:562–567

    Google Scholar 

  18. Aerssens J, Van Audekercke R, Geusens P, Schot L, Abdel-Hamid Osman A, Dequeker J (1993) Mechanical properties, bone mineral content, and bone composition (collagen, osteocalcin, IGF-I) of the rat femur: influence of ovariectomy and nandrolone decanoate (anabolic steroid) treatment. Calcif Tissue Int 53:269–277

    Google Scholar 

  19. Bouillon R, Van Baelen H, De Moor P (1977) The measurement of the vitamin D-binding protein in human serum. J Clin Endocrinol 45:225–237

    Google Scholar 

  20. Verhaeghe J, Van Herck E, Visser WJ, Suiker AMH, Thomasset M, Einhorn TA, Faierman E, Bouillon R (1990) Bone and mineral metabolism in BB rats with long-term diabetes. Decreased bone turnover and osteoporosis. Diabetes 39:249–255

    Google Scholar 

  21. Bouillon R, Vanderschueren D, Van Herck E, Nielsen HK, Bex M, Heyns W, Van Baelen H (1992) Homologous radioimmunoassay of human osteocalcin. Clin Chem 38:2055–2060

    Google Scholar 

  22. Gevers G, Dequeker J, Geusens P, Nyssen-Behets C, Dhem A (1989) Physical and histomorphological characteristics of iliac crest bone differ according to grade of osteoarthritis of the hand. Bone 10:173–178

    Google Scholar 

  23. Raymaekers G, Aerssens J, Van Den Eynde R, Peeters J, Guesens P, Devos P, Dequeker J (1992) Alterations of the mineralization profile and osteocalcin concentrations in osteoarthritic cortical iliac crest bone. Calcif Tissue Int 51:269–275

    Google Scholar 

  24. Turner RT Puza JE, Forte MD, Lester GE, Gray TK, Howard GA, Baylink DJ (1980) In vitro synthesis of 1 α,25-dihydroxy-cholecalciferol and 24,25-dihydroxycholecalciferol by isolated calvarial cells. Biochemistry 77:5720–5724

    Google Scholar 

  25. Howard GA, Turner RT, Sherrard DJ, Baylink DJ (1981) Human bone cells in culture metabolize 25-hydroxyvitamin D3 to 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. J Biol Chem 256:7738–7774

    Google Scholar 

  26. Turner RT, Howard GA, Puzas JE, Baylink DJ, Knapp DR (1983) Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3. Biochemistry 22:1073–1076

    Google Scholar 

  27. Ichikawa F, Sato K, Nanjo M, Nishii Y, Shinki T, Takahashi N, Suda T (1995) Mouse primary osteoblasts express vitamin D3 25-hydroxylase mRNA and convert 1α-hydroxyvitamin D3 into 1,25-dihydroxyvitamin D3. Bone 16:129–135

    Google Scholar 

  28. Boivin G, Mesguich P, Pike JW, Bouillon R, Meunier PJ, Hausler MR, Dubois PM, Morel G (1987) Ultrastructural immunocytochemical localization and endogenous 1,25-dihydroxyvitamin from neonatal mouse and rat calvaria. Bone Miner 3:125–126

    Google Scholar 

  29. Canalis E, McCarthy T, Centrella M (1989) The role of growth factors in skeletal remodeling. Endocrinol Metab Clin North Am 18:903–918

    Google Scholar 

  30. Bianco P, Rimunucci M, Bonucci E, Termine JD, Robey PG (1993) Bone sialoprotein (BSP) secretion and osteoblast differentiation: relationship to bromodeoxyuridine incorporation, alkaline phosphatase, and matrix deposition. J Histochem Cytochem 41:183–191

    Google Scholar 

  31. Lian JB, Roufosse AH, Reit B, Glimcher MJ (1982) Concentrations of osteocalcin and phosphoprotein as a function of mineral content and age in cortical bone. Calcif Tissue Int 34:S82-S87

    Google Scholar 

  32. Canalis E (1993) Insulin-like growth factors and the local regulation of bone formation. Bone 14:273–276

    Google Scholar 

  33. Hock J, Centrella M, Canalis E (1988) Insulin-like growth factor I has independent effects on bone matrix formation and cell replication. Endocrinology 122:254–260

    Google Scholar 

  34. Farley JR, Tarbaux N, Murphy LA, Masuda T, Baylink DJ (1987) In vitro evidence that bone formation may be coupled to resorption by release to mitogen(s) from resorbing bone. Metabolism 36:314–321

    Google Scholar 

  35. Raisz G (1990) Local and systemic factors in the pathogenesis of osteoporosis. N Engl J Med 318:818–828

    Google Scholar 

  36. Bouillon R, Van Assche F, Van Baelen H, Heyns W, De Moor P (1981) Influence of the vitamin D-binding protein on the serum concentration of 1,25-dihydroxyvitamin D3. Significance of the free 1,25-dihydroxyvitamin D3 concentration. J Clin Invest 67:589–596

    Google Scholar 

  37. Quesada J, Coopmans W, Ruiz R, Aljama P, Jans I, Bouillon R (1992) Influence of vitamin D on parathyroid function in the elderly. J Clin Endocrinol Metab 75:494–501

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Experimental Medicine and Endocrinology, Onderwijs en Navorsing, B-3000, Leuven, Belgium

    I. Jans, E. Van Herck, I. Vandewal & R. Bouillon

  2. Arthritis and Metabolic Bone Disease Research Unit, U.Z. Gasthuisberg, Belgium

    S. Boonen, J. Aerssens, J. Peeters, R. Van den Eynde & J. Dequeker

  3. Department of Internal Medicine, Division of Geriatric Medicine, U.Z. Gasthuisberg, Belgium

    S. Boonen

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  1. S. Boonen
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Boonen, S., Aerssens, J., Jans, I. et al. Lack of In vitro evidence for storage of 1,25-dihydroxycholecalciferol (1,25(OH)2D3) and 1,25(OH)2D3 binding protein in skeletal matrix. Calcif Tissue Int 59, 488–491 (1996). https://doi.org/10.1007/BF00369215

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  • DOI: https://doi.org/10.1007/BF00369215

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