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II. Enhancement of gas transport in inositol hexaphosphate-loaded red blood cells

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Abstract

The gas-transport function of red blood cells which have incorporated inositol hexaphosphate is significantly improved by fusion with effector-loaded lipid vesicles. “Right-shifts” of the O2-binding curves of inositol hexaphosphate-loaded red blood cells with half-saturation pressures at 37 °C up to 98 mmHg are observed. The transformation of 56% of the intracellular haemoglobin into the low affinity state corresponds to an optimum increase of the O2-release capacity of 270%. The CO2 transport is also correspondingly enhanced. The treated red blood cells show no inositol hexaphosphate depletion during a reasonable time.

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Authors and Affiliations

  1. Abteilung für Physiologische Chemie der Technischen Hochschule, D-5100, Aachen

    Klaus Gersonde

  2. Institut für Strahlenchemie im Max-Planck-Institut für Kohlenforschung, D-4330, Mülheim (Ruhr)

    Claude Nicolau

Authors
  1. Klaus Gersonde
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  2. Claude Nicolau
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Additional information

Excellent assistance from D. Schüller, H. Dittrich, and V. Hiltenkamp is gratefully acknowledged.

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Gersonde, K., Nicolau, C. II. Enhancement of gas transport in inositol hexaphosphate-loaded red blood cells. Naturwissenschaften 66, 567–570 (1979). https://doi.org/10.1007/BF00368811

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  • DOI: https://doi.org/10.1007/BF00368811

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