Skip to main content
Log in

Transformation of murine LMTK cells with purified HLA class I genes

III. Human HLA class I antigens coded by hybrid genes constructed in vitro indicate association of serologic reactivities with the first two domains of the molecule

  • Brief Communications
  • Published:
Immunogenetics Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Barbosa, J. A., Kamarck, M. E., Biro, P. A., Weissman, S. M., and Ruddle, F. H.: Identification of human genomic clones coding the major histocompatibility antigens HLA-A2 and HLA-B7 by DNAmediated gene transfer. Proc. Natl. Acad. Sci. U.S.A. 79: 6327–6331, 1982

    Google Scholar 

  • Colbere-Garapin, F., Chousterman, S., Horodniceanu, F., Kourilsky, P., and Garapin, A.-C.: Cloning of the active thymidine kinase gene of herpes simplex virus type I in Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 76: 3755–3759, 1979

    Google Scholar 

  • Evans, G. A., Margulies, D. H., Shykind, B., Seidman, J. G., and Ozato, K.: Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. Nature 300: 755–757, 1982

    Google Scholar 

  • Hood, L., Steinmetz, M., and Malissen, B.: Genes of the major histocompatibility complex of the mouse. Anna. Rev. Immunol. 1, in press, 1983

  • Jordan, B. R., Bregegère, F., and Kourilsky, P.: Human HLA gene segment isolated by hybridization with mouse H-2 cDNA probes. Nature 290: 521–523, 1981

    Google Scholar 

  • Lemonnier, F. A., Malissen, M., Golstein, P., Le Bouteiller, P., Rebai, N., Damotte, M., Birnbaum, D., Caillol, D., Trucy, J., and Jordan, B. R.: Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L cells. Immunogenetics 16: 355–361, 1982

    Google Scholar 

  • Lemonnier, F. A., Le Bouteiller. P., Malissen, B., Golstein, P., Malissen, M., Mishal, Z., Caillol, D. J., Jordan, B. R., and Kourilsky, F. M.: Transfection of murine LMTK- cells with purified HLA class I genes. I. Modification of conformation of murine β 2-microglobulin upon its association with HLA heavy chains. J. Immunol., in press, 1983a

  • Lemonnier, F. A., Dubreuil, P. C., Layet, C., Malissen, M., Bourel, D., Mercier, P., Jakobsen, B. K., Caillol, D. H., Svejgaard, A., Kourilsky, F. M., and Jordan, B: R.: Transformation of LMTK- cells with purified HLA class I genes. II. Serological characterization of HLA-A3 and -CW3 molecules. Immunogenetics, in press, 1983b

  • Malissen, M., Malissen, B., and Jordan, B. R.: Exon/Intron organization and complete nucleotide sequence of an HLA gene. Proc. Natl. Acad. Sci. U.S.A. 79: 893–897, 1982a

    Google Scholar 

  • Malissen, M., Damotte, M., Birnbaum, D., Trucy, J., and Jordan, B. R.: HLA cosmid clones show complete, widely spaced class I genes with occasional clusters. Gene 20: 485–489, 1982b

    Google Scholar 

  • Margulies, D. H., Evans, G. A., Ozato, K., Camerini-Otero, R. D., Tanaka, K., Appella, E., and Seidman, J. G.: Expression of H-2Dd and H-2d d mouse major histocompatibility antigen genes in L cells after DNA-mediated gene transfer. J. Immunol. 130: 463–470, 1983

    Google Scholar 

  • Singer, D. S., Camerini-Otero, R. D., Satz, M. L., Osborne, B., Sachs, D., and Rudikoff, S.: Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells. Proc. Natl. Acad. Sci. U.S.A. 79: 1403–1407, 1982

    Google Scholar 

  • Steinmetz, M., Moore, K. V., Frelinger, J. G., Sher, B. T., Shen, F.-W., Boyse, E. A., and Hood, L.: A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell 25: 683–692, 1981

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jordan, B.R., Lemonnier, F.A., Caillol, D.H. et al. Transformation of murine LMTK cells with purified HLA class I genes. Immunogenetics 18, 165–171 (1983). https://doi.org/10.1007/BF00368546

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00368546

Navigation