Abstract
IgE levels after Nippostrongylus brasiliensis infestation were high in all strains of mice examined except in the SJA/9 strain. However, surface IgE levels on B cells were not different from those observed in the SJL strain. Most surface IgE-bearing cells also had surface IgM molecules. We suggest that the surface IgE-bearing cells are precursors of IgE-producing cells. In adoptive transfer, hapten-specific IgE antibody was produced when hapten-primed cells from SJA/9 mice were transferred together with cells from SJL donors infested with N. brasiliensis but not when the cells were from SJA/9 donors. It is suggested that the lack of collaboration between hapten-primed B cells and cells from infested mice is a genetic trait of the SJA/9 strain.
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Abbreviations
- KLH:
-
keyhole limpet hemocyanin
- DNP:
-
dinitrophenyl
- Nb:
-
Nippostrongylus brasiliensis extract
- PBS:
-
phosphate buffered saline
- SDS:
-
sodium dodecyl sulfate
- FITC:
-
fluorescein-isothiocyanate
- RITC:
-
rhodamine-isothiocyanate
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Kumagai, Y., Hirano, T., Watanabe, N. et al. Studies on IgE production in mice. Immunogenetics 18, 147–153 (1983). https://doi.org/10.1007/BF00368543
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DOI: https://doi.org/10.1007/BF00368543