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Partial reconstruction of the lampbrush loop pair Nooses on the Y chromosome of Drosophila hydei

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Abstract

We present the analysis of genomic DNA fragments that were isolated as potential segments of the lampbrush loop pair Nooses on the short arm of the Y chromosome of Drosophila hydei. More than 300 kb of DNA were recovered in BamHI lambda and cosmid clone groups. This DNA is composed of the Y-specific ayl family of repetitive DNA sequences, and of other repetitive DNA sequences, which at least in part are also located elsewhere in the genome (Y-associated sequences). Two additional classes of DNA fragments were obtained from an EcoRI libary. One of them consists of ayl repeats without apparent interspersion, including a total of more than 300 kb of DNA. The other is composed of tandemly repeated YsI sequences, a Y-specific sequence derived from ayl. This class includes more than 400 kb of DNA, which is also not interspersed by other sequences. Our results show that only the ayl repeats interspersed by Y-associated DNA sequences can represent parts of the 260 kb transcription unit forming the lampbrush loop, whereas the ayl and YsI repeats without interspersion form separate and nontranscribed clusters of repetitive DNA.

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Author notes

  1. Andy Pötgens

    Present address: Institute of Pathology, University Hospital Nijmegen, Geert Grooteplein Zuid 24, NL-6525 GA, Nijmegen, The Netherlands

  2. Hans Meijer

    Present address: Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné Weg 10, D-50820, Köln, Germany

  3. Rosilde Dijkhof

    Present address: Department of Animal Breeding, Zodiac, Agricultural University, Marijkeweg 40, NL-6709 PG, Wageningen, The Netherlands

Authors and Affiliations

  1. Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands

    Ron Hochstenbach, Andy Pötgens, Hans Meijer, Rosilde Dijkhof, Miriam Knops, Karin Schouren & Wolfgang Hennig

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  1. Ron Hochstenbach
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  2. Andy Pötgens
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Communicated by: E. Schmidt

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Hochstenbach, R., Pötgens, A., Meijer, H. et al. Partial reconstruction of the lampbrush loop pair Nooses on the Y chromosome of Drosophila hydei . Chromosoma 102, 526–545 (1993). https://doi.org/10.1007/BF00368346

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  • DOI: https://doi.org/10.1007/BF00368346

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