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Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin

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Abstract

Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA eluted at higher temperature from chromatin than from purified DNA. One explanation for the higher melting temperature of mouse satellite DNA in chromatin suggests that the complex between this particular DNA component and at least some proteins in chromatin is more stable than the average DNA-protein interaction.

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References

  1. McConaughy, B. L. and McCarthy, B. J., Biochemistry 11, 998 (1972).

    Google Scholar 

  2. Reeck, G. R., Arch. Biochem. Biophys. 172, 117 (1976).

    Google Scholar 

  3. Corneo, G., Ginelli, E., Soave, C., and Bernardi, G., Biochemistry 7, 4373 (1968).

    Google Scholar 

  4. Dawid, I. B., Brown, D. D., and Reeder, R. H., J. Mol. Biol. 51, 341 (1970).

    Google Scholar 

  5. Sinclair, J. H. and Brown, D. D., Biochemistry 10, 2761 (1971).

    Google Scholar 

  6. Reeder, R. H., and Brown, D. D., J. Mol. Biol. 51, 361 (1970).

    Google Scholar 

  7. Brown, D. D. and Weber, C. S., J. Mol. Biol. 34, 661 (1968).

    Google Scholar 

  8. Burton, K., Biochem. J., 62, 315 (1956).

    Google Scholar 

  9. Ansevin, A. T., Hnilica, L. S., Spelsberg, T. C., and Keham, S. L., Biochemistry 10, 4793 (1971).

    Google Scholar 

  10. Reeck, G. R., Simpson, R. T. and Sober, H. A., Proc. Nat. Acad. Sci. U.S.A. 69, 2317 (1972).

    Google Scholar 

  11. Sponar, J. and Sormova, Z., Eur. J. Biochem. 29, 99 (1972).

    Google Scholar 

  12. Hwan, J. C., Leffak, I. M., Li, H. J., Huang, P. C., and Mura, C., Biochemistry 14, 1390 (1975).

    Google Scholar 

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Author notes

  1. Iliya G. Pashev

    Present address: Institute of Biochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria

Authors and Affiliations

  1. Department of Embryology, Carnegie Institution of Washington, 115W. University Pkwy, 21210, Baltimore, Maryland, USA

    Iliya G. Pashev

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  1. Iliya G. Pashev
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Pashev, I.G. Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin. Molecular Biology Reports 3, 297–303 (1977). https://doi.org/10.1007/BF00368300

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  • DOI: https://doi.org/10.1007/BF00368300

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