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Comparative Haematology International

, Volume 5, Issue 3, pp 183–188 | Cite as

Possible occurrence of amitotic cell division during haemopoiesis in the Urodeles

  • S. Barni
  • A. Fraschini
  • E. Prosperi
  • R. Vaccaronel
  • F. Bernini
Original Article

Abstract

The liver haemopoietic activity of three species of Urodeles (Triturus carnifex, Triturus alpestris and Speleomantes ambrosii) was examined by morphocytochemical approaches (light and electron microscopy, anti-BrdU immunocytochemistry, flow cytometry). The proliferation of haemopoietic cells, detected by the anti-BrdU labelling index, was accompanied by absence of mitotic cell division and the appearance of cells showing features of amitosis (e.g. nuclear constrictions with bundles of electron-dense chromatin) sometime positive to the anti-BrdU immuno-gold reaction. The possible unbalanced segregation of chromatin during the direct division of the nucleus was detected by flow cytometric measurement in terms of heterogeneous relative DNA content in peripheral blood cells. The presence in the bloodstream samples of cells (erythrocytes) with replicating DNA, nuclear constrictions and binucleations is also consistent with a situation of direct nuclear division.

Keywords

Amitosis Haemopoiesis Liver Urodeles Morpho-cytochemistry Erythrocytes 

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Copyright information

© Springer-Verlag London Limited 1995

Authors and Affiliations

  • S. Barni
    • 1
  • A. Fraschini
    • 1
  • E. Prosperi
    • 2
  • R. Vaccaronel
    • 1
  • F. Bernini
    • 1
  1. 1.Dipartimento di Biologia AnimaleUniversità di PaviaItaly
  2. 2.Centro di Studio per l'Istochimica del C.N.R.PaviaItaly

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