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Characterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L.

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Abstract

A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 which complemented MLC640. Further subcloning and site-directed Tn5 mutagenesis localized the gene for nodule development to a 1.7-kb region within the 3.6-kb EcoRI fragment. Southern hybridization using the 3.6-kb EcoRI fragment as the probe against genomic DNA of several Rhizobium spp. indicated that this gene is conserved in different rhizobia.

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Authors
  1. S. Pooyan
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  2. M. L. C. George
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  3. D. Borthakur
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Additional information

The authors are with the Department of Plant Molecular Physiology, University of Hawaii, 3050 Maile Way, Gimore 402, Honolulu, Hawaii 96822. USA;

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Pooyan, S., George, M.L.C. & Borthakur, D. Characterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L.. World J Microbiol Biotechnol 10, 583–589 (1994). https://doi.org/10.1007/BF00367673

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  • DOI: https://doi.org/10.1007/BF00367673

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