Abstract
Unsheared DNA has been isolated from Rhodotorula and Rhodosporidium yeasts using a cell-wall-digesting enzyme preparation from Paecilomyces lilacinus. Pulsed-field gel electrophoresis indicated that at least 11 chromosomes were present in Rhodot. gracilis ATCC 90950. The DNA was amenable to digestion with restriction enzymes.
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The authors are with the Department of Microbiology, Central Food Technological Research Institute, Mysore-570 013, India.
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Vishnuvardhan, D., Joseph, R. A simple method for isolation of high-molecular-weight DNA from Rhodotorula gracilis . World Journal of Microbiology & Biotechnology 11, 291–293 (1995). https://doi.org/10.1007/BF00367101
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DOI: https://doi.org/10.1007/BF00367101