Abstract
1(2)-Dehydrogenation of 4-cholestene-3-one by immobilized Mycobacterium fortuitum NRRL B-8153 and free growing Mycobacterium sp. NRRL B-3805 and Micromonospora chlacea MTCC 329 depended on the nature of the C-17 side chain. The 1(2)-dehydrogenation did not appear to occur before C-17-side-chain cleavage of the 4-cholestene-3-one.
Similar content being viewed by others
References
ArimaK., NagasawaM., BaeM. & TamuraG. 1969 Microbial transformations of sterols. Part I. Decomposition of cholesterol by microorganisms. Agricultural and Biological Chemistry 33, 1636–1643.
ConstantinidesA. 1980 Steroid transformation at high substrate concentration using immobilized Corynebacterium simplex cells. Biotechnology and Bioengineering 22, 119–136.
Goswami, P.C., Singh, H.D. & Baruah, J.N. 1984 Factors limiting the microbial conversion of sterols to 17-ketosteroids in presence of metal chelate inhibitors. Folia Microbiologia 29, 209–216.
HocknullM.D. & LillyM.D. 1990 The use of free and immobilized Arthrobacter simplex in organic solvent/aqueous two-liquid phase reactors. Applied Microbiology and Biotechnology 33, 147–153.
LarssonP.O. & MosbachK. 1976 Immobilization of steroid-transforming organisms in polyacrylamide. Methods in Enzymology 44, 183–190.
MartinC.K.A. 1977 Microbial cleavage of sterol side chain. Advances in Applied Microbiology 22, 29–58.
MillerT.L. 1985 Steroid fermentations. In Comprehensive Biotechnology, Vol. 3, eds BlanchH.W., DrewS. & WangD.I.C. pp. 297–318. New York: Pergamon Press.
NagasawaM., BaeM., TamuraG. & ArimaK. 1969 Microbial transformations of sterols. Part II. Cleavage of sterol side-chains by microorganisms. Agricultural and Biological Chemistry 33, 1644–1650.
NagasawaM., WatanabeN., HashibaH., TamuraG. & ArimaK. 1970 Microbial transformation of sterols. Part III. Substrate specificity for cleaving steroid side chain by Arthrobactor simplex. Agricultural and Biological Chemistry 34, 798–800.
OhlsonS., LarssonP.O. & MosbachK. 1978 Steroid transformation by activated living immobilized Arthrobacter simplex cells. Biotechnology and Bioengineering 20, 1267–1284.
PatilS., SrivastavaA., ShuklaA. & PhaseN. 1991 Spectrophotometric estimation of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione during C-1(2)-dehydrogenation by Mycobacterium fortuitum NRRL B-8153. World Journal of Microbiology and Biotechnology 7, 626–627.
PhaseN. & PatilS. 1994 Natural oils are better than organic solvents for the conversion of soybean sterols to 17-ketosteroids by Mycobacterium fortuitum. World Journal of Microbiology and Biotechnology 10, 228–229.
SihC.J. & WangK.C. 1965 A new route to estrone from sterols. Journal of the American Chemical Society 87, 1387–1388.
WovchaM.G., AntoszF.J., KnightJ.C., KomineckL.A. & PykeT.R. 1978 Bioconversion of sitosterol to useful steroidal intermediates by mutants of Mycobacterium fortuitum. Biochimica et Biophysica Acta 531, 308–321.
Additional information
S. Patil is and A. Srivastava was with the School of Life Sciences, Devi Ahilya Vishwavidyalaya. Vigyan Bhawan, Khandwa Road Campus, Indore-452 001, India. A. Srivastava is now with the Shripati Singhania R&D Centre. J. K. Pharmaceuticals. 13th Mile Stone. Mathura Road, Faridabad (Haryana) 121 003, India.
Rights and permissions
About this article
Cite this article
Srivastava, A., Patil, S. Dependence of steroid 1(2)-dehydrogenation on the C-17 side chain during cholesterol metabolism by immobilized Mycobacterium fortuitum . World Journal of Microbiology & Biotechnology 11, 284–286 (1995). https://doi.org/10.1007/BF00367099
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00367099