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Isolation of the yeast INO4 gene, a positive regulator of phospholipid biosynthesis

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Summary

Yeast ino4 mutants are auxotrophic for the phospholipid precursor inositol and have pleiotropic defects in phospholipid synthesis. The mutants are unable to derepress the cytoplasmic enzyme, inositol-1-phosphate synthase and they exhibit reduced synthesis of methylated phospholipids, particularly phosphatidylcholine. The INO4 gene is believed to encode a positive regulator involved in coordinate control of phospholipid synthesis. In the present study, we report the isolation of two clones containing the INO4 gene. The clones share a region of homology and were mapped to the INO4 locus. Southern blot analysis revealed that the cloned DNA contained both unique and repetitive DNA.

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Author notes

  1. Lisa S. Klig

    Present address: Department of Biological Sciences, Stanford University, Stanford, CA

  2. Deborah K. Hoshizaki

    Present address: Department of Biological Chemistry, College of Medicine, University of Illinois at Chicago, 1853 West Polk Street, 60612, Chicago, IL, USA

Authors and Affiliations

  1. Department of Genetics and Molecular Biology, Albert Einstein College of Medicine, 10461, Bronx, NY, USA

    Lisa S. Klig & Deborah K. Hoshizaki

  2. Department of Biological Sciences, Carnegie Mellon University, 15213, Pittsburgh, PA, USA

    Deborah K. Hoshizaki

Authors
  1. Lisa S. Klig
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  2. Deborah K. Hoshizaki
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Klig, L.S., Hoshizaki, D.K. Isolation of the yeast INO4 gene, a positive regulator of phospholipid biosynthesis. Curr Genet 13, 7–14 (1988). https://doi.org/10.1007/BF00365749

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  • DOI: https://doi.org/10.1007/BF00365749

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