Summary
We have determined DNA sequences of the mating factor a genes of Saccharomyces uvarum and Saccharomyces italicus and compared them to that of the MFα1 gene of S. cerevisiae. The DNA sequences of the mating factor genes in both species were almost completely identical to that of the MFα1 gene of S. cerevisiae except for the number of tandem repeated units; these latter consisted of a spacer peptide and a mature mating factor and there were three units in S. uvarum and five units in S. italicus compared with four units in the MFα1 of S. cerevisiae. From the detailed comparison of DNA sequences of the spacer peptide-mating factor units from these three species, the high sequence homology can be recognized in the internal units of the tandem repeats. This suggests that the internal units might be multiplied preferentially in the tandem repeated units of mating factor genes.
Similar content being viewed by others
References
Brake AJ, Julius DJ, Thorner J (1983) Mol Cell Biol 3:1440–1450
Cryer DR, Eccleshall R, Marmur J (1975) Methods Cell Biol 12:39–44
Dmochowska A, Dignard D, Henning D, Thomas DY, Bussey H (1987) Cell 50:573–584
Jackson JA, Fink GR (1985) Genetics 109:303–332
Julius D, Schekman R, Thorner J (1984) Cell 36:309–318
Kurjan J (1985) Mol Cell Biol 5:787–796
Kurjan J, Herskowitz I (1982) Cell 30:933–943
Maloney DH, Fogel S (1987) Curr Genet 12:1–7
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Maxam AM, Gilbert W (1980) Methods Enzymol 65:499–560
Sakurai A, Sakata K, Tamura S, Aizawa K, Yanagishima N, Shimoda C (1976) Agric Biol Chem 40:1057–1058
Singh A, Chen EY, Lugovoy JM, Chang CN, Hitzeman RA, Seeburg PH (1983) Nucleic Acids Res 11:4049–4063
Southern E (1975) J Mol Biol 98:503–517
Stotzler D, Kiltz H, Duntze W (1976) Eur J Biochem 69:397–400
Wallace RB, Johnson MJ, Hirose T, Miyake T, Kawashima EH, Itakura K (1981) Nucleic Acids Res 9:879–894
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kitada, K., Hishinuma, F. Evidence for preferential multiplication of the internal unit in tandem repeats of the mating factor a genes in Saccharomyces yeasts. Curr Genet 13, 1–5 (1988). https://doi.org/10.1007/BF00365748
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00365748