Summary
A procedure has been developed for the rapid isolation of yeast nuclei in high yield using Percoll gradients. The nuclei are substantially free of cytoplasmic contamination as measured by alcohol dehydrogenase activities, have the typical chromatin digestion pattern when digested with nucleases, are useful for isolation of nuclear proteins and for in vitro transcription experiments.
Similar content being viewed by others
References
Bhargava MM, Halvorson HO (1971) J Cell Biol 49:423–429
Bonner WM, Laskey RA (1974) Eur J Biochem 46:83–88
Buitrago A, Gylfe E, Henriksson C, Pertoft H (1977) Biochem Biophys Res Comm 79:823–828
Davie JR, Saunders CA, Walsh JM, Weber SC (1981) Nucleic Acids Res 9:3205–3216
De Kloet SR, Beltz WR (1975) Arch Biochem Biophys 167:322–334
Dietz E, Hamann K (1976) Ang. Makromol. Chemie 51:53–60
Duffus JH (1969) Biochim Biophys Acta 195:230–233
Groner B, Phillips S (1975) J Biol Chem 250:5640–5646
Haff LA (1979) Prep Biochem 9:149–156
Hendriks AW (1972) FEBS Lett 24:101–105
Ide GJ (1981) Biochemistry 20:2633–2638
Klingeborn B, Pertoft H (1972) J Neurochem 19:811–823
Laemmli UK (1970) Nature 227:680–685
Loening VE (1967) Biochem J 102:251
Lohr D, Van Holde KE (1975) Science 188:165–166
Lohr D, Kovacic RT, Van Holde KE (1977a) Biochemistry 16:463–471
Lohr D, Tatchell K, Van Holde KE (1977b) Cell 12:829–836
Lohr D, Corden J, Tatchell K, Kovacic RT, Van Holde KE (1977c) Proc Natl Acad Sci USA 74:79–83
Lohr D, Ide G (1979) Nucleic Acids Res 6:1909–1927
Loir M, Wyrobek A (1972) Exp Cell Res 75:261–268
Maniatis T, Jeffrey A, van de Sande H (1975) Biochemistry 14:3787–3793
Mardian JKW, Isenberg I (1978) Biochemistry 17:3825–3833
May R (1971) Z Allg Mikrobiol 11:131–142
Noll M (1974) Nucleic Acids Res 1:1573–1578
Pertoft H (1970a) Virology 41:368–372
Pertoft H (1970b) Anal Biochem 38:506–516
Pertoft H, Rubin K, Kjellen L, Laurent TC, Klingeborn B (1977) Exp Cell Res 110:449–458
Pertoft H, Laurent TC, Laas T, Kågedal L (1978) Anal Biochem 88:271–282
Reeve AE, Smith MM, Piegiet V, Huang RCC (1977) Biochemistry 16:4464–4469
Rozijn TH, Tonimo GJM (1964) Biochim Biophys Acta 91:105–112
Sajdel-Sulkowska EM, Bhargava MM, Arnard MV, Halvorson HO (1974) Biochem Biophys Res Commun 56:496–502
Schmitt JM, Herrmann RG (1977) Methods in Cell Biol 15:177–200
Schultz LD (1978) Biochemistry 17:750–758
Takabe T, Nishimura, M, Akazawa T (1979) Agric Biol Chem 43:2137–2142
Tekamp PA, Valenzuela P, Maynard T, Bell GI, Rutter WJ (1979) J Biol Chem 254:955–963
Vallee BL, Hoch FL (1955) Proc Natl Acad Sci USA 41:327–338
Wintersberger V, Smith P, Letnansky K (1973) Eur J Biochem 33:123–130
Wolff DA (1975) Methods in Cell Biology 10:85–104
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ide, G.J., Saunders, C.A. Rapid isolation of yeast nuclei. Current Genetics 4, 85–90 (1981). https://doi.org/10.1007/BF00365686
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365686