Summary
Data presented here demonstrate that fusion of protoplasts of two different haploid strains of Saccharomyces cerevisiae having the same mating type leads to the formation of “fusants” and “cytoplasmic hybrids”. The nuclear and cytoplasmic genome of a “fusant” combine those of the parent haploid strains. The “cytoplasmic hybrid” possesses the haploid genome of one parent and the combined cytoplasmic genomes of both. In mouse cells lines such products have been termed “cybrids” and this term has therefore been adopted here (Bunn and Wallace 1974).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allmark BM, Morgan AJ, Whittaker PA (1978) Mol Gen Genet 159:297–299
Arima K, Takano I (1979) Mol Gen Genet 173:271–279
Bevan EA, Somers J (1969) Genet Res Camb 14:71–77
Bevan EA, Herring AJ, Mitchell DJ (1973) Nature 255:81–86
Bunn CL, Wallace DC (1974) Proc Natl Acad Sci USA 71:1681–1692
Clark-Walker GD (1972) Proc Natl Acad Sci USA 69:388–392
Cox BS, Bevan EA (1961) Trans Br Mycol Soc 44:239–342
Ferenczy L, Maraz A (1977) Nature 268:524–525
Guerineau M, Slonimski P, Avner PR (1974) Biochem Biophys Res Commun 61:462–469
Gunge N, Sakaguchi K (1979) Mol Gen Genet 170:243–247
Gunge N, Tamura A (1978) Jpn J Genet 53:50–53
Lancashire WE, Matoon JR (1979) Mol Gen Genet 170:33–34
Livingston DM (1977) Genetics 86:73–84
Livingston DM, Kupfer DM (1977) b J Mol Biol 116:244–260
Maraz A, Kiss M, Ferenczy L (1978) FEMS Microbiol Letters 3:319–322
Morgan AJ, Brunner A, Whittaker PA (1980) Curr Genet 2:87–93
Ortega-Ruiz JM (1977) Microbial Letters 4:133–137
Pomper S, Davids KM, McKnee DW (1954) Genetics 39:343–355
Sherman F, Lawrence CW (1974) Handbook of genetics 1. Plenum Press, New York, pp 359–393
Sipiczki M, Ferenczy L (1977) Mol Gen Genet 151:77–81
Slonimski P, Perrodin G, Croft JM (1968) Biochem Biophys Res Commun 30:232–239
Solingen P, van der Plaat JB (1977) J Bacteriol 130:946–947
Stahl U (1978) Mol Gen Genet 113:111–113
Svoboda A (1978) J Gen Microbiol 109:169–175
Yamamoto M, Fukui S (1977) Agric Biol Chem 31:1829–1830
Zakaharov I, Yurchenko LV, Yaravoy P (1969) Genetika 5:136–141
Zakharov I, Yaravoy P (1977) Mol Cell Biochem 14:14–18
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goodey, A.R., Bevan, E.A. Production and genetic analysis of yeast cybrids. Current Genetics 7, 69–72 (1983). https://doi.org/10.1007/BF00365683
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365683