Summary
We investigated the heat shock response of the adenylate cyclase deficient mutant cr-1 (crisp) of Neurospora crassa. This strain was observed to be much more resistant to a lethal temperature of 50 °C than the wild type. This constitutive thermotolerance was absent in cr-1 conidiospores raised on cyclic AMP (cAMP, 2.5 mM) supplemented solid medium, but was partially restored when the conidiospores were germinated at 30 °C, a temperature which fails to induce thermotolerance in the wild-type strain. Two other crisp-like Neurospora mutants, cr-2 and cr-3 which, in contrast to cr-1, contain normal levels of cAMP, did not exhibit the thermotolerance phenomenon observed for cr-1. A cr-1, pe, fl (crisp-microconidial) strain also lacked the ability to tolerate a lethal heat treatment. Our results demonstrate that the adenylate cyclase deficient cr-1 mutant of Neu-rospora crassa expresses a constitutive thermotolerant phenotype as a consequence of its primary genetic defect: low levels of cAMP.
Similar content being viewed by others
References
Barrat RW, Ogata WN (1972) Neurospora Newslett 19:34–105
Brockman HE, De Serres FJ (1963) Am J Bot 50:709–714
Cortat M, Turian G (1974) Arch Microbiol 95:305–309
Garnjobst L, Tatum EL (1970) Genetics 66:281–290
Jorge JA, Terenzi HF (1980) Develop Biol 74:231–238
Kapoor M, Lewis J (1987) Can J Microbiol 33:162–168
Pall ML, Trevillyan JM, Hinman N (1981) Mol Cell Biol 1:1–8
Perkins DD (1959) Genetics 44:1185–1208
Plesofsky-vig N, Brambl R (1985) J Bacteriol 162:1083–1091
Rosenberg G, Pall ML (1979) J Bacteriol 137:1140–1144
Said S, Terenzi HF (1981) Braz J Med Biol Res 14:3–10
Shin D-Y, Matsumoto K, Iida H, Uno I, Ishikawa T (1987) Mol Cell Biol 7:244–250
Terenzi HF, Flawia MM, Torres HN (1974) Biochem Biophys Res Commun 58:990–996
Terenzi HF, Flawia MM, Tellez-Inon MT, Torres HN (1976) J Bacteriol 126:91–99
Terenzi HF, Jorge JA, Roselino JE, Migliorini RH (1979) Arch Microbiol 123:251–258
Torres HN, Flawia MM, Terenzi HF, Tellez-Inon MT (1975) In: Brummond GI, Greengard P, Robison GA (eds) Advances in cyclic nucleotide research, vol 5. Raven Press, New York, pp 67–78
Vogel HJ (1956) Microbiol Genet Bull 13:42–43
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cruz, A.K., Terenzi, H.F., Jorge, J.A. et al. Cyclic AMP dependent, constitutive thermotolerance in the adenylate cyclase-deficient cr-1 (crisp) mutant of Neurospora crassa . Curr Genet 13, 451–454 (1988). https://doi.org/10.1007/BF00365668
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00365668