Summary
We have cloned a mutant oligomycin resistance allele of the mitochondrial ATP synthase subunit 9 gene from the filamentous fungus Penicillium chrysogenum. The gene was isolated using the equivalent gene from Aspergillus nidulans as a hybridisation probe. Using the cloned gene it is possible to select for oligomycin resistance in P. chrysogenum transformation experiments. This transformation system was used to introduce further copies of the P. chrysogenum isopenicillin N synthetase gene, which were stably maintained without selection. An assessment of the frequency with which homologous integration occurs was also made. With this system, it should prove possible to transform any strain of P. chrysogenum.
Similar content being viewed by others
References
Ballance DJ, Buxton FP, Turner G (1983) Biochem Biophys Res Commun 112:284–289
Beri R, Turner G (1987) Curr Genet 11:639–641
Binninger DM, Skrzynia C, Pukkila PJ, Casselton LA (1987) EMBO J 6:835–840
Buxton FP, Radford A (1984) Mot Gen Genet 196:339–344
Carr LG, Skatrud PL, Scheetz II ME, Queener SW, Ingolia TD (1987) Gene 48:257–266
Casadaban MJ, Cohen SN (1980) J Mot Biol 138:179–207
Case ME, Schweizer M, Kushner SR, Giles NH (1979) Proc Natl Acad Sci USA 76:5259–5263
Enns RK, Criddle RS (1977) Arch Biochem Biophys 182:587–600
Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) J Mot Biol 170:827–842
Johnstone IL, Hughes SG, Clutterbuck AJ (1985) EMBO J 4: 1307–1311
Kelly JM, Hynes MJ (1985) EMBO J 4:475–479
Kinnaird JH, Keighren MA, Kinsey JA, Eaton M, Fincham JRS (1982) Gene 20:387–396
Maniatis T, Fritsch EF, Sambrook (1982) Molecular Cloning. (A laboratory manual) Cold Spring Harbor Laboratory, Cold Spring Harbor
Paietta J, Marzluf GA (1985) In: Timberlake WE (ed) Molecular genetics of filamentous fungi. Liss, New York
Picknet TM, Saunders G, Ford P, Holt G (1987) Curr Genet 12: 449–455
Orbach MJ, Porro E, Yanofsky C (1986) Mot Cell Biol 6:2452–2461
Queener SW, Ingolia TD, Skatrud PL, Chapman JL, Kaster KR (1985) In: Schlesinger D (ed) Microbiology. Am Soc Microbiol, pp 468–472
Rodriguez RJ, Yoder OC (1987) Gene 54:73–81
Rowlands RT, Turner G (1973) Mol Gen Genet 126:201–216
Samson SM, Belagaje R, Blankenship DT, Chapman JL, Perry D, Skatrud PL, VanFrank RM, Abraham EP, Baldwin JE, Queener SW, Ingolia TD (1985) Nature 318:191–194
Sanchez F, Tourino A, Trasiera S, Peres-Aranda A, Rubio V, Penalva MA (1987) Gene 51:97–102
Sebald W, Sebald-Althaus M, Wachter E (1977) In: Bandlow W, Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1977 — genetics and biogenesis of mitochondria. de Gruyter, Berlin
Skatrud PL, Queener SW, Carr LG, Fisher DL (1987) Curr Genet 12:337–348
Tilburn J, Scazzocchio C, Taylor GG, Zabricky-Zissman JH, Lockington RA, Davies RW (1983) Gene 26:205–221
Turner G, Imam G, Kuntzel H (1979) Fur J Biochem 97:565–571
Viera J, Messing J (1982) Gene 19:259–268
Ward M, Wilkinson B, Turner G (1986) Mot Gen Genet 202:265–270
Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bull, J.H., Smith, D.J. & Turner, G. Transformation of Penicillium chrysogenum with a dominant selectable marker. Curr Genet 13, 377–382 (1988). https://doi.org/10.1007/BF00365658
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365658