Summary
We started partial overlapping hybridization on chromosome V from the centromere-linked gene SU8 towards centromere 5. Transformation of Podospora anserina with the cloned sequences and subsequent genetic analysis reveal that cosmids issuing from a 60 kb region never integrate near centromere 5, whereas cosmids from either side return mostly to this locus. Molecular analysis shows that heterologous integrations of cosmids spanning the 60 kb region occurs in a specific sequence of 11 kb common to all the inserts. We propose that this sequence contains a gene or a structure which is lethal in a duplicated form. Straightforward interpretation of genetic data suggests that this structure does not correspond to centromere 5. Its function is still unknown.
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References
Brygoo Y, Debuchy R (1985) Mol Gen Genet 200:128–131
Carbon J (1984) Cell 37:351–353
Case ME (1986) Genetics 113:569–587
Clarke L, Amstutz H, Fishel B, Carbon J (1986) Proc Natl Acad Sci USA 83:8253–8257
Coppin-Raynal E (1982) Curr Genet 5:57–63
Coppin-Raynal E, Le Coze D (1982) Genet Res 40:149–164
Covarrubias L, Bolivar F (1982) Genet 17:79–89
Dani GM, Zakian VA (1983) Proc Natl Acad Sci USA 80:3406–3410
Debuchy R, Brygoo Y (1985) EMBO J 4:3553–3556
Esser K (1974) In: King RC (ed) Handbook of genetics. Plenum Press, NY, pp 531–551
Hohn B (1975) J Mol Biol 98:93–106
Hohn B, Collins J (1980) Gene 11:291–298
Ish-Horowicz D, Burke JF (1981) Nucleic Acids Res 9:2989–2998
Lambie EJ, Roeder GS (1986) Genetics 114:769–789
Maniatis T, Fritsch EF, Sambroo J (1982) Molecular cloning. A loboratory manual. Cold Spring Harbor Laboratory Press, NY
Marcou D, Picard-Bennoun M, Simonet JM (1984) In: O'Brien SJ (ed) Genetics maps 1984, vol 3. Cold Spring Harbor Laboratory, NY, pp 252–261
Mortimer RK, Schild D (1985) Microbiol Rev 49:181–212
Nakaseko Y, Adachi Y, Funahashi S, Niwa O, Yanagida M (1986) EMBO J 5:1011–1021
Perrot M, Barreau C, Bégueret J (1987) Mol Cell Biol 7:1725–1730
Picard M, Debuchy R, Julien J, Brygoo Y (1987) Mol Gen Genet 210:129–134
Rizet G, Engelmann C (1949) Rev Cytol Biol Veg 11:201–304
Seed B, Parker RC, Davidson N (1982) Gene 19:201–209
Sreekrishna K, Webster TD, Dickson RC (1984) Gene 28:73–81
Strathern JN, Newlon CS, Herskowitz I, Hicks JB (1979) Cell 18:309–319
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Debuchy, R., Coppin-Raynal, E., Le Coze, D. et al. Chromosome walking towards a centromere in the filamentous fungus Podospora anserina: cloning of a sequence lethal at a two-copy state. Curr Genet 13, 105–111 (1988). https://doi.org/10.1007/BF00365644
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DOI: https://doi.org/10.1007/BF00365644