Skip to main content

Mitochondrial DNA from Podospora anserina

IV. The large ribosomal RNA gene contains two long intervening sequences

Current Genetics volume 7, pages 151–157 (1983)Cite this article

Summary

We have examined the structure of the rRNA genes from the mitochondrial genome of Podospora anserina. Using R-loop analysis, nuclease protection experiments, and Southern blot hybridization analysis we have observed two intervening sequences (IVS) in the large rRNA gene, and none in the small rRNA gene. the IVS sequences are 1.65 kbp and 2.73 kbp long, and the larger of the two is in the position of the conserved IVS found in the mitochondrial genomes of other fungi. We have detected precursor transcripts for the large rRNA, and these data support the observation of two IVS in this gene. We also note that the large and small rRNA genes are separated by approximately 6 kbp of DNA.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Bailey JM, Davidson N (1976) Anal Biochem 70:75–85

    Google Scholar 

  • Berk AJ, Sharp P (1977) Cell 12:721–732

    Google Scholar 

  • Bertrand H, Bridge P, Collins RA, Garriga G, Lambowitz AM (1982) Cell 29:517–526

    Google Scholar 

  • Boerner P, Mason TL, Fox TD (1981) Nucleic Acids Res 9:6379–6390

    Google Scholar 

  • Bolivar F (1978) Gene 4:121–136

    Google Scholar 

  • Bonitz SG, Coruzzi G, Thalenfeld BE, Tzagoloff A, Macino G (1980) J Biol Chem 255:11927–11941

    Google Scholar 

  • Bos JL, Osinga KA, Von Der Horst G, Hecht NB, Tabak HF, Van Ommen GJB, Borst P (1980) Cell 20:207–214

    Google Scholar 

  • Broker TR, Soll L, Chow LT (1977) J Mol Biol 113:579–589

    Google Scholar 

  • Cummings DJ, Belcour L, Grandchamp C (1979) Mol Gen Genet 171:229–238

    Google Scholar 

  • Davies RW, Scazzocchio C, Waring RB, Lee S, Grisi E, Berks MM, Brown TA (1982) In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial Genes: Cold Spring Harbor Monographs

  • Dujon B (1980) Cell 20:185–197

    Google Scholar 

  • Grant DM, Lambowitz AM (1982) In the Cell Nucleus, Busch H, Rothblum L (eds), vol X. Academic Press, New York, NY

    Google Scholar 

  • Gray MW, Doolittle WF (1982) Microbiol Rev 46:1–42

    Google Scholar 

  • Grimm MF, Lambowitz AM (1979) J Mol Biol 134:667–672

    Google Scholar 

  • Green MR, Grimm MF, Goewert RR, Collins RA, Cole MD, Lambowitz AM, Heckman JE, Yin S, Raj Bhandary AL (1981) J Biol Chem 256:2027–2034

    Google Scholar 

  • Grunstein M, Hogness DS (1975) Proc Natl Acad Sci USA 72:3961–3965

    Google Scholar 

  • Hahn U, Lazarus CM, Lunsdorf H, Kuntzel H (1979) Cell 17:191–200

    Google Scholar 

  • Heckman JE, Raj Bhandary UL (1979) Cell 17:583–595

    Google Scholar 

  • Kuntzel H, Kochel HG, Lazarus CM, Lunsdorf H (1982) In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial Genes. Cold Spring Harbor Monographs 12

  • Lazarus CM, Lunsdorf H, Han U, Stepien PP, Kuntzel H (1980) Mol Gen Genet 177:389–397

    Google Scholar 

  • Lazowska J, Jacq C, Slonimski PP (1980) Cell 22:333–348

    Google Scholar 

  • Learned RM, Meyers RM, Tjian R (1981) In: Ray DS (ed) The Initiation of DNA Replication. Academic Press, New York, pp 555–566

    Google Scholar 

  • Mannella CA, Collins RA, Green MR, Lambowitz AM (1979) Proc Natl Acad Sci USA 76:2635–2639

    Google Scholar 

  • Merten S, Synenki RM, Locker J, Christianson T, Rabinowitz M (1980) Proc Natl Acad Sci USA 77:1417–1421

    Google Scholar 

  • Rigby PW, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:239–251

    Google Scholar 

  • Rosbash M, Blank D, Fahrner K, Hereford L, Riccardi R, Roberts B, Ruby S, Woolford T (1979) Meth Enzymol 68:454–469

    Google Scholar 

  • Smith JR, Rubenstein I (1973) J Gen Microbiol 76:297–304

    Google Scholar 

  • Southern EM (1975) J Mol Biol 98:503–517

    Google Scholar 

  • Thomas P (1980) Proc Natl Acad Sci USA 77:5201–5205

    Google Scholar 

  • Villarreal LP, White RI, Berg P (1979) J Virol 29:209–219

    Google Scholar 

  • Wallace DC (1982) Microbiol Rev 46:208–240

    Google Scholar 

  • Weaver RF, Weissman C (1979) Nucleic Acids Res 7:1175–1193

    Google Scholar 

  • Wright RM, Laping JL, Horrum MA, Cummings DJ (1982a) Mol Gen Genet 185:56–64

    Google Scholar 

  • Wright RM, Horrum MA, Cummings DJ (1982b) Cell 29:505–515

    Google Scholar 

Download references

Author information

Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, University of Colorado, Health Sciences Center, Campus Box B-175, 80262, Denver, Colorado, USA

    Richard M. Wright & Donald J. Cummings

Authors
  1. Richard M. Wright
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Donald J. Cummings
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wright, R.M., Cummings, D.J. Mitochondrial DNA from Podospora anserina . Current Genetics 7, 151–157 (1983). https://doi.org/10.1007/BF00365641

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00365641

Key words

  • Podospora anserina
  • Mitochondrial DNA
  • Ribosomal Genes
  • Intervening Sequences
  • Transcripts