Two of the main reasons for developing serum-free medium are to improve the growth and to increase the productivity of a cell. This review has attetmpted to show that these goals can be achieved far more easily than generally realised by utilising the methods that have been developed during the past decade and by using a limited number of standard supplements. Serum-free media have contributed greatly to the advances made in recent years in the fields of cell biology and mammalian cell technology and it is clear that they will continue to play a major role in the clarification of cellular regulatory processes and in the refinement of processes in modern cyto-technology.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Avner ED, Sweney WEJr and Ellis D (1984) Serum-free organ culture of embryonic mouse metanephros. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol. 3 (pp. 33–41), Alan R. Liss, Inc., New York.
Barnes DW (1984) Serum-free culture of A431 human epidermoid carcinoma. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol.3 (pp. 95–106), Alan R. Liss, Inc., New York.
Barnes DW (1987) Serum-free animal cell culture. BioTechniques, 5: 534–542.
Blasey HD and Winzer U (1989) Low protein serum-free medium for antibody production in stirred bioreactors. Biotechnol. Lett. 11: 455–460.
Bödeker BGD, Berg GJ, Steiner U, Hewlett G and Schlumberger HD (1983) J. Tiss. Cult. Meth. 8: 155–159.
Bödeker BGD, Berg GJ, Hewlett G and Schlumberger HD (1985) Dev. Biol. Standard 60: 3–100.
Büntemeyer H (1988) Entwicklung eines Perfusionssystems zur kontinuierlichen Kultivierung tierischer Zellen in Suspension. Thesis for Dr. rer. nat., University of Hannover.
Butler M (1986) Serum-free media. In: Thilly WG (ed.) Mammalian Cell Technology. (pp. 91–108) Butterworths, Boston.
Clark JM, Gebb C and Hirtenstein MD (1981) Serum supplements and serum-free medium: applicability for microcarrier culture of animal cells. Develop. Biol. Standard. 50: 193–198.
Glassy MC, Tharakan JP and Chau PC (1988) Serum-free media in hybridoma culture and monoclonal antibody production. Biotech. Bioeng. 32: 1015–1028.
Gospodarowicz D (1984) Preparation and uses of lipoproteins to culture normal diploid and tumor cells under serum-free conditions. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology. Vol. 1 (pp. 69–86) Alan R. Liss, Inc., New York.
Greenwood D, Srinivasan A, McGoogan S and Pipas JM (1989) Effects of oncogene expression on cellular growth factor requirements: defined media for the culture of C3H 10T1/2 and NIH3T3 cell lines. In: Baserga R (ed.) Cell Growth and Division a practical approach (pp. 37–59) IRL Press, Oxford.
Gürhan SI and Özdural N (1990) Serial cultivation of suspended BHK21/13 cells in serum-reduced and serum-free medium supplemented with various membrane protective agents. Cytotechnology 3: 89–93.
Ham RG (1981) Survival and growth requirements of nontransformed cells. Handbook Exp. Pharmacol. 57: 13–88.
Ham RG (1984) Growth of human fibroblast cultures in serum-free media. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol. 3 (pp. 249–264), Alan R. Liss, Inc., New York.
Handa-Corrigan A, Emery AN and Spier RE (1989) Effect of gas-liquid interfaces on growth of suspended mammalian cells: mechanisms of cell damage by bubbles. Enzyme Microb. Technol. 11: 230–235.
Hewlett G (1989) Aprotinin Funktion und Wirkung in der biomedizinischen Forschung und Entwicklung. BioEngineering 5/89: 40–42.
Hewlett G, Duvinski MS and Montalto JG (1989) Pentex Ex-Cyte growth enhancement media supplement as a lipoprotein additive for mammalian cell culture. Miles Sci. J. 11: 9–14.
Ill CR, Brehm T, Lyderson BK, Hernandez R and Burnett KG (1988) Species specificity of iron delivery in hybridomas. In Vitro Cell. Dev. Biol. 24: 413–419.
Jäger V, Lehmann J and Friedl P (1988) Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors. Cytotechnology 1: 319–329
Kan M and Yamane I (1982) In vitro proliferation of human diploid fibroblasts in serum-free BSA-containing medium. J. Cell. Physiol. 111: 155–162.
Katsuta H, Takaoka T, Hosaka S, Hibino M, Otsuki I, Hattori K, Suzuki S and Mitamura K (1959) Protein requirements of rat ascites hepatoma cells in tissue culture. Jpn. J. Exp. Med. 29: 45–70.
MacLeod AJ (1988) The use of plasma protein fractions as medium supplements for animal cell culture. Adv. Biochem. Eng. 37: 41–56.
Maurer HR (1986) Towards chemically-defined, serum-free media for mammalian cell culture. In: Freshney RI (ed.) Animal Cell Culture a practical approach. (pp. 13–31) IRL Press, Oxford.
Metzger JF, Fusilio MH, Cornman I and Kuhns DM (1954) Antibiotics in tissue culture. Exp. Cell. Res. 6: 337–344.
Mizrahi A (1975) Pluronic polyols in human lymphocyte cell line cultures. J. Clin. Microbiol. 2: 11–13.
Moellering WJ, Tedesco JL, Townsend RR, Hardy MR, Scott RW and Prior CP (1990) Electrophoretic differences in a MAb expressed in three media. Biopharm. 3: 30–38.
Murakami H, Okazaki Y, Yamada K and Omura H (1988) Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium. Cytotechnology 1: 159–169.
Murakami H (1990) What should be focused in the study of cell culture technology for production of bioactive proteins. Cytotechnology 3: 3–7.
Murhammer DW and Goochee CF (1988) Scale-up of insect cell cultures: protective effects of pluronic F68. Beta-galactoside production in Spodoptera frugiperda culture. Bio/ Technology 6: 1411–1415.
Paul J (1959) Cell and Tissue Culture. E & S Livingstone Ltd., Edinburgh.
Riss TL, Karey KP, Burleigh BD, Parker D and Sirbasku DA (1988) Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using Balb/c 3T3 mouse embryo fibroblasts. In Vitro Cell. Dev. Biol. 24: 1099–1106.
Rockwell GA, (1984) Growth of SV40 Balb/c-3T3 cells in serum-free culture medium. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol. 3 (pp. 221–231), Alan R. Liss, Inc., New York.
Saier MHJr (1984) Hormonally defined, serum-free medium for a proximal tubular kidney epithelial cell line, LLC-PK1. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol. 3 (pp. 25–31), Alan R. Liss, Inc. New York.
Shintani Y, Iwamoto K and Kitano K (1988) Polyethylene glycols for promoting the growth of mammalian cells. Appl. Microbiol. Biotechnol. 27: 533–537.
Swim HE and Parker RF (1960), Effect of pluronic F68 on growth of fibroblasts in suspension on a rotary shaker. Proc. Sci. Exp. Biol. Med. 103: 252–254.
Taub M (1984) Growth of primary and established kidney cell cultures in serum-free media. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol. 3 (3–24), Alan R. Liss, Inc., New York.
Walthall BJ and Ham RG (1981) Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin and dexamethasone. Exp. Cell Res. 134: 303–311.
Waymouth C (1984) Preparation and use of serum-free culture media. In: Barnes DW, Sirbasku DA and Sato GH (eds.) Cell Culture Methods for Molecular and Cell Biology, Vol. 1 (pp. 23–68), Alan R. Liss, Inc., New York.
About this article
Cite this article
Hewlett, G. Strategies for optimising serum-free media. Cytotechnology 5, 3–14 (1991). https://doi.org/10.1007/BF00365530