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Establishment and characterization of a new human colon adenocarcinoma cell line: BCS-TC2

Cytotechnology volume 3, pages 75–88 (1990)Cite this article

Abstract

A new cell line designated as BCS-TC2 was established in culture from a primary human colon adenocarcinoma. This cell line has been in continuous culture over a 36-month period. The cells grow as a monolayer sheet, displaying areas with a multilayered pattern as well as single cells and free-floating aggregates. The morphological, immunological, and ultrastructural features of these cells are in agreement with their epithelial origin. The characterization of this cell line indicated a 38 hr doubling time, and a colony forming efficiency of 2% in semisolid media and 22% in liquid culture, at low cell densities. These cells produce low amounts of carcinoembryonic antigen in culture (0.1 ng of CEA/106 cells). Sub-cutaneous injection into athymic mice shows that these cells have a non-tumorigenic capacity. Chromosomal analysis showed a karyotype 46 XX,-15, +der (15), inv (16) (p13::q13). BCS-TC2 cell line, which maintains in culture several characteristics of the original tumor, represents a useful model system for cell biology studies of primary and non-metastatic tumors.

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Authors and Affiliations

  1. Dpto. Bioquímica y Biología Molecular I, Universidad Complutense, 28040, Madrid, Spain

    Javier Turnay, Nieves Olmo, José G. Gavilanes & Maria A. Lizarbe

  2. Dpto. Genética Humana, Fundación Jiménez Díaz, 28040, Madrid, Spain

    Javier Benitez

Authors
  1. Javier Turnay
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  2. Nieves Olmo
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  3. José G. Gavilanes
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  4. Javier Benitez
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  5. Maria A. Lizarbe
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Turnay, J., Olmo, N., Gavilanes, J.G. et al. Establishment and characterization of a new human colon adenocarcinoma cell line: BCS-TC2. Cytotechnology 3, 75–88 (1990). https://doi.org/10.1007/BF00365268

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  • DOI: https://doi.org/10.1007/BF00365268

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