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A set of 4 nuclear proteins binds to a DNA sequence within the FOS promoter region

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Abstract

We investigated DNA-protein-interactions occurring in the promoter region of c-fos using two-dimensional electrophoresis and south-western-blotting. When nuclear extracts from the human glioblastoma cell line HeRoSV were tested for their DNA-binding behaviour to a 650 bp-fragment within the promoter region of c-fos, we found 4 proteins designated as 120/6.6, 75/5.4, 65/6.4 55/5.0 interacting with this fragment. An additional protein 60/6.0 was detected by using a digoxygenine-labelled probe. These observations let us to assume that beside the well characterized SRF and FOS-JUN proteins additional factors recognize the promoter sequence and may play a role in c-fos regulation.

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Abbreviations

DRE:

direct repeat element

DSE:

dyad symmetry element

DTE:

Dithiothreitol

EGF:

Epidermal growth factor

FCS:

fetal calf serum

PA:

polyacrylamide

PMSF:

Phenylmethylsulfonyl fluoride

SDS:

Sodiumdodecylsulfate

SRF:

serum response factor

TCF:

ternary complex factor

TPA:

12-O-tetradecanoyl phorbol-13-acetate

2D:

two-dimensional

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Authors and Affiliations

  1. Institute of Human Genetics, University of the Saar, D-6650, Hamburg-Saar, Germany

    Janna Radtke, Steven Dooley, Nikolaus Blin & Gerhard Unteregger

Authors
  1. Janna Radtke
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  2. Steven Dooley
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  3. Nikolaus Blin
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  4. Gerhard Unteregger
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Radtke, J., Dooley, S., Blin, N. et al. A set of 4 nuclear proteins binds to a DNA sequence within the FOS promoter region. Mol Biol Rep 15, 87–92 (1991). https://doi.org/10.1007/BF00364844

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  • DOI: https://doi.org/10.1007/BF00364844

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