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Polytene chromosome sof monogenic and amphogenic Chrysomya species (Calliphoridae, diptera): analysis of banding patterns and in situ hybridization with Drosophila sex determining gene sequences

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Abstract

Standard maps for the five banded polytene chromosomes found in trichogen cell nuclei of the monogenic blowfly Chrysomya rufifacies and the amphogenic Chrysomya pinguis are presented. The chromosomes are highly homologous in the two species; differences in banding patterns are predominantly caused by one pericentric and ten paracentric inversions. In chromosome 5 of the amphogenic Chrysomya phaonis, also analysed in this paper, an additional paracentric inversion was observed. The distribution of species specific inversions indicates that the monogenic C. rufifacies is phylogenetically older than the amphogenic species. The maternal sex realizer locus F'/f on polytene chromosome 5 of C. rufifacies is not associated with a structural heterozygosity. Chromosome pair 6 of C. rufifacies and the sex chromosome pair of C. pinguis are under-replicated in polytene nuclei; they consist of irregular chromatin granules, frequently associated with nucleolus material. Evolution of heteromorphic sex chromosomes in Chrysomya is probably correlated with heterochromatin accumulation. A search for sex determining genes in Chrysomya was initiated using sex determining sequences from Drosophila melanogaster for in situ hybridization. The polytene band 41A1 on chromosome 5 of monogenic and amphogenic Chrysomya species contains sequences homologous to the maternal sex determining gene daughterless (da). Homology to the zygotic gene Sex-lethal (Sxl) of Drosophila is detected in band 39A1 on chromosome 5 of C. rufifacies. The findings reported here are the first evidence for a possible homology between the da gene of Drosophila and the maternal sex realizer F′ of C. rufifacies. An hypothesis for the evolution of the maternal effect sex determination of C. rufifacies is proposed.

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References

  • Ananiev EV, Barsky VE, Ilyin YV, Churikov NA (1981) Localization of nucleoli in Drosophila melanogaster polytene chromosomes. Chromosoma 81:619–628

    Google Scholar 

  • Ashburner M, Carson HL, Thompson JN (eds) (1982) The genetics and biology of Drosophila, vol 3b. Academic Press, London

    Google Scholar 

  • Beckingham K, Thompson N (1982) Under-replication of intron+ rDNA cistrons in polyploid nurse cell nuclei of Calliphora erythrocephala. Chromosoma 87:177–196

    Google Scholar 

  • Bedo DG (1991) Cytological characterization of heterochromatin in meiotic and mitotic chromosomes of the Old World screwworm fly, Chrysomya bezziana (Diptera: Calliphoridae). Genome 34:631–637

    Google Scholar 

  • Bedo DG (1992a) Polytene chromosomes of the Old World screwworm fly (Chrysomya bezziana) and its evolutionary relationships with Lucilia cuprina and Cochliomyia hominivorax (Diptera: Calliphoridae). Genome 35:294–303

    Google Scholar 

  • Bedo DG (1992b) Nucleolar fragmentation in polytene trichogen cells of Lucilia cuprina and Chrysomya bezziana (Diptera: Calliphoridae). Genome 35:283–293

    Google Scholar 

  • Bell LR, Maine EM, Schedl P, Cline TW (1988) Sex-lethal, a Drosophila sex determination switch gene, exhibits sex-specific RNA splicing and sequence similarity to RNA binding proteins. Cell 55:1037–1046

    Google Scholar 

  • Belote JM (1992) Sex determination in Drosophila melanogaster: from the X:A ratio to doublesex. Dev Biol 3:319–330

    Google Scholar 

  • Boon DJ (1975) Comparative study of genetics of Calliphoridae. Bachelor of Science Thesis, Australian National University, Canberra, ACT, Australia

  • Bopp D, Bell LR, Cline TW, Schedl P (1991) Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster. Genes Dev 5:403–415

    Google Scholar 

  • Bull JJ (1987) Sex determining mechanisms: An evolutionary perspecitive. In: Stearns SC (ed) The evolution of sex and its consequences. Brikhäuser Verlag, Basel-Boston

    Google Scholar 

  • Carson HL, Yoon JS (1982) Genetics and evolution of Hawaiian Drosophila. In: Ashburner M, Carson HL, Thompson JN (eds) The genetics and biology of Drosophila, vol 3b. Academic Press, London

    Google Scholar 

  • Caudy M, Vässin H, Brand M, Tuma R, Jan LY, Jan YN (1988a) Daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex. Cell 55:1061–1067

    Google Scholar 

  • Caudy M, Grell EH, Dambly-Chaudière C, Ghysen A, Jan LY, Jan YN (1988b) The maternal sex determination gene daughterless has zygotic activity necessary for the formation of peripheral neurons in Drosophila. Genes Dev 2:843–852

    Google Scholar 

  • Childress D (1969) Polytene chromosomes and linkage group-chromosome correlations in the Australian sheep blowfly Lucilia cuprina (Diptera: Calliphoridae). Chromosoma 26:208–214

    Google Scholar 

  • Cline TW (1983) The interaction between daughterless and Sexlethal in triploids: A lethal sex transforming maternal effect linking sex determination and dosage compensation in Drosophila melanogaster. Dev Biol 95:260–274

    Google Scholar 

  • Cronmiller C, Cline TW (1986) The relationship of relative gene dose to the complex phenotype of the daughterless locus in Drosophila. Dev Genet 7:205–221

    Google Scholar 

  • Cronmiller C, Cline TW (1987) The Drosophila sex determination gene daughterless has different functions in the germ line versus the soma. Cell 48:479–487

    Google Scholar 

  • Cronmiller C, Schedl PU, Cline TW (1988) Molecular characterization of daughterless, a Drosophila sex determination gene with multiple roles in development. Genes Dev 2:1666–1676

    Google Scholar 

  • Dev V, LaChance LE, Whitten CJ (1986) Polytene chromosomes, karyotype correlations, and population cytology of the primary screwworm fly. J Hered 77:427–434

    Google Scholar 

  • Endow SA, Glover DM (1979) Differential replication of ribosomal gene repeats in polytene nuclei of Drosophila. Cell 17:597–605

    Google Scholar 

  • Foster GG, Whitten MJ, Konovalov C, Bedo DG, Maddern RH, Boon DJ (1980) Cytogenetic studies of Lucilia cuprina dorsalis R.-D. Polytene chromosome maps of the autosomes and cytogenetic localization of visible genetic markers. Chromosoma 81:151–168

    Google Scholar 

  • Gasch A, Hinz U, Leiss D, Renkawitz-Pohl R (1988) The expression of β1 and β3 tubulin genes of Drosophila melanogaster is spatially regulated during embryogenesis. Mol Gen Genet 211:8–16

    Google Scholar 

  • Gergen JP (1987) Dosage compensation in Drosophila: evidence that daughterless and Sex-lethal control X chromosome activity at the blastoderm stage of embryogenesis. Genetics 117:477–485

    Google Scholar 

  • Hayashi S, Gillam IC, Delaney AD, Tener GM (1978) Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA. J Histochem Cytochem 26:677–679

    Google Scholar 

  • Keyl HG (1962) Chromosomenevolution bei Chironomus. II. Chromosomenumbauten und phylogenetische Beziehungen der Arten. Chromosoma 13:464–514

    Google Scholar 

  • Kirchhoff C (1988) GATA tandem repeats detect minisatellite regions in blowfly DNA (Diptera: Calliphoridae). Chromosoma 96:107–111

    Google Scholar 

  • Maine EM, Salz HK, Cline TW, Schedl P (1985) The Sex-lethal gene of Drosophila: DNA alterations associated with sex-specific lethal mutations. Cell 43:521–529

    Google Scholar 

  • Mange AP, Sandler L (1973) A note on the maternal effect mutants daughterless and abnormal oocyte in Drosophila melanogaster. Genetics 73:73–86

    Google Scholar 

  • Murre C, Schonleber McCraw P, Baltimore D (1989) A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell 56:777–783

    Google Scholar 

  • Newman LJ (1977) Chromosomal evolution of the Hawaiian Telmatogeton (Chironomidae, Diptera). Chromosoma 64:349–369

    Google Scholar 

  • Nöthiger R, Steinmann-Zwicky M (1985) A single principle for sex determination in insects. Cold Spring Habor Symp Quant Biol 50:615–621

    Google Scholar 

  • Ribbert D (1967) Die Polytänchromosomen der Borstenbildungszellen von Calliphora erythrocephala. Unter besonderer Berücksichtigung der geschlechtsgebundenen Strukturheterozygotie und des Puffmusters während der Metamorphose. Chromosoma 21:296–344

    Google Scholar 

  • Rothfels KH (1979) Cytotaxonomy of black flies (Simuliidae). Annu Rev Entomol 24:507–539

    Google Scholar 

  • Rothfels KH (1980) Chromosomal variability and speciation in blackflies. In: Insect cytogenetics. Symposium of the Royal Entomological Society of London, vol 10, pp 207–224

  • Rothfels KH (1989) Speciation in black flies. Genome 32:500–509

    Google Scholar 

  • Schöttke M (1986) Chromosomenverhältnisse und konstitutives Heterochromatin bei einigen Calliphoriden (Diptera: Calliphoridae). Diplomarbeit, Universität Kiel

  • Ullerich F-H (1963) Geschlechtschromosomen und Geschlechtsbestimmung bei einigen Calliphorinen (Calliphoridae, Diptera). Chromosoma 14:45–110

    Google Scholar 

  • Ullerich F-H (1973) Die genetische Grundlage der Monogenie bei der Schmeißfliege Chrysomya rufifacies (Calliphoridae, Diptera). Mol Gen Genet 125:157–172

    Google Scholar 

  • Ullerich F-H (1975) Identifizierung der genetischen Geschlechtschromosomen bei der monogenen Schmeißfliege Chrysomya rufifacies (Calliphoridae, Diptera). Chromosoma 50:393–419

    Google Scholar 

  • Ullerich F-H (1976) Chromosomenverhältnisse, konstitutives Heterochromatin und Geschlechtsbestimmung bei einigen Arten der Gattung Chrysomya (Calliphoridae, Diptera). Chromosoma 58:113–136

    Google Scholar 

  • Ullerich F-H (1984) Analysis of sex determination in the monogenic blowfly Chrysomya rufifacies by pole cell transplantation. Mol Gen Genet 193:479–487

    Google Scholar 

  • White MJD (1973) Animal cytology and evolution. Cambridge University Press

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  1. Zoologisches Institut der Universität Kiel, Biologiezentrum, Olshausenstrasse 40, D-24098, Kiel, Germany

    Susanne Puchalla

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  1. Susanne Puchalla
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Dedicated to Professor Dr. Fritz-Helmut Ullerich on the occasion of his 60th birthday

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Puchalla, S. Polytene chromosome sof monogenic and amphogenic Chrysomya species (Calliphoridae, diptera): analysis of banding patterns and in situ hybridization with Drosophila sex determining gene sequences. Chromosoma 103, 16–30 (1994). https://doi.org/10.1007/BF00364722

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  • DOI: https://doi.org/10.1007/BF00364722

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