Summary
In order to test the applicability of the occlusion time method for measuring the reabsorptive characteristics in the proximal convolution of the rat kidney, a quantitative analysis of this method was performed. The time course of decrease in tubular diameter, following interruption of GFR, was measured from time sequenced photomicrographs taken before and during the period of occlusion. Tubular occlusion, as measured by direct microscopic observation or by extrapolating the initial slope of decrease in luminal diameter to zero diameter, was found to be constant over a range of GFR from 0.3–2.2 ml/min · g kidney weight. A positive correlation was observed between GFR and free flow luminal diameter. The decrease in luminal diameter, which occurs during the period of occlusion, is a linear function of time.
The results indicate that the lateral water flux is directly related to the free flow luminal diameter prior to the period of occlusion, i.e., to GFR. In contrast, the lateral water flux remains constant during the decrease in luminal diameter, which occurs during the period of occlusion. Although the results are consistent with the assumption of a glomerular-tubular balance in the proximal convolution, the absolute values for lateral water flux, as measured by the occlusion method, are approximately twice as high as values calculated from free flow micropuncture experiments, split droplet experiments, and cinematographic experiments.
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Literature
Brandis, M., G. Braun u. K. H. Gertz: Retrograder Flüssigkeitsstrom in kortikale Tubuli bei Aortenabklemmung. 5. Symp. Ges. Nephrol., Lausanne 1967.
Brunner, F. P., F. C. Rector, and D. W. Seldin: Mechanism of glomerulotubular balance. II Regulation of proximal tubular reabsorption by tubular volume, as studied by stopped flow microperfusion. J. clin. Invest. 45, 603 (1966).
Führ, J., J. Kaczmarczyk u. C. D. Krüttgen: Eine einfache Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin. Wschr. 33, 729 (1955).
Gerlach, E., W. Bader u. W. Schwoerer: Über den Stoffwechsel säurelöslicher Phosphatverbindungen. Pflügers Arch. ges. Physiol. 272, 407 (1961).
Gertz, K. H.: Transtubuläre Natriumchloridflüsse und Permeabilität für Nichtelektrolyte im proximalen und distalen Konvolut der Rattenniere. Pflügers Arch. ges. Physiol. 276, 336 (1963).
—, G. Braun, and H. D. Pagel: On the glomerular-tubular balance in the rat kidney. Pflügers Arch. ges. Physiol. 285, 360 (1965).
Hierholzer, K., M. Wiederholt u. H. Stolte: Hemmung der Natriumresorption im proximalen und distalen Konvolut adrenalektomierter Ratten. Pflügers Arch. ges. Physiol. 291, 43 (1966).
Horster, M., W. Nagel, J. Schnermann u. K. Thurau: Zur Frage einer direkten Angiotensinwirkung auf die Natriumresorption im proximalen Tubulus und in der Henleschen Schleife der Rattenniere. Pflügers Arch. ges. Physiol. 292, 118 (1966).
Landwehr, D. M., R. M. Klose, and G. Giebisch: Renal tubular sodium and water reabsorption in the isotonic sodium loaded rat. Amer. J. Physiol. 212, 1327 (1967).
Leyssac, P. P.: Dependence of glomerular filtration rate on proximal tubular reabsorption of salt. Acta physiol. scand. 58, 236 (1963).
Rector, F. C., F. P. Brunner, and D. W. Seldin: Mechanism of glomerulotubular balance. I. Effect of aortic constriction and elevated ureteropelvic pressure on glomerular filtration rate, fractional reabsorption, transit time, and tubular size in the proximal tubule of the rat. J. clin. Invest. 45, 590 (1966).
Solomon, S., and J. C. Vanatta: Implication of phospholipids in rat proximal tubule reabsorption. Proc. Soc. exp. Biol. (N. Y.) 122, 1040 (1966).
Steinhausen, M.: Eine Methode zur Differenzierung proximaler und distaler Tubuli der Nierenrinde von Ratten in vivo und ihre Anwendung zur Bestimmung tubulärer Strömungsgeschwindigkeiten. Pflügers Arch. ges. Physiol. 277, 23 (1963).
Thurau, K., u. P. Deetjen: Kinematographische Untersuchungen am Warmblüternephron. Nachr. Akad. Wissensch., Göttingen 27 (1961).
Wiederholt, M., K. Hierholzer, E. E. Windhager, and G. Giebisch: Microperfusion study of fluid reabsorption in proximal tubules of kidneys. Amer. J. Physiol. 213, 809 (1967).
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Supported by the Deutsche Forschungsgemeinschaft and the US Department of the Army, through its European Research Office.
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Wahl, M., Nagel, W., Fischbach, H. et al. On the application of the occlusion time method for measurements of lateral net fluxes in the proximal convolution of the rat kidney. Pflügers Archiv 298, 141–153 (1967). https://doi.org/10.1007/BF00364694
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DOI: https://doi.org/10.1007/BF00364694