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Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by α1 and α2 but not α3 external domains

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Abstract

We have shown that cytotoxic T lymphocytes (CTL) raised in H-2 dmice use H-2Ld but not H-2Dd or H-2Kd antigens as restricting elements in lymphocytic choriomeningitis virus (LCMV) and vesicular stomatis virus (VSV) infections. To localize the regions of H-2Ld protein recognized by CTL, we constructed a recombinant H-2L d/D dgene encoding a hybrid antigen with α1 and α2 external domains of H-2Ld and α3, transmembrane and cytoplasmic domains of H-2Dd. The recombinant gene was transfected into mouse cells and the hybrid molecules were characterized serologically, biochemically and functionally. In all assays, H-2Ld/Dd molecules were recognized by LCMV- and VSV-specific H-2Ld-restricted CTL in a manner similar to that of wild-type H-2Ld antigens. Analogous results were obtained with alloreactive CTL. Hybrid antigens containing the α3 domain of H-2Ld fused to α1 and α2 domains of a Qa-2,3 region-encoded antigen were not used as restricting elements by LCMV-specific CTL. These results suggest that H-2Ld-restricted CTL directed against LCMV and VSV recognize determinants controlled by the α1 and/or α2 domains of the H-2Ld molecule.

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Abbreviations

CTL:

cytotoxic T lymphocytes

VSV:

vesicular stomatitis virus

LCMV:

lymphocytic choriomeningitis virus

tk:

thymidine kinase

HAT:

hypoxanthine, aminopterine, thymidine

HSV:

herpes simplex virus

FCS:

fetal calf serum

SAC:

Staphylococcus aureus Cowan I strain

TM:

transmembrane

CYT:

cytoplasmic

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Author notes

  1. Robert S. Goodenow

    Present address: Department of Genetics, University of California, 94720, Berkeley, California

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, 91125, Pasadena, California

    Iwona Stroynowski, Anders Orn, Robert S. Goodenow & Leroy Hood

  2. Department of Microbiology, University of Southern California School of Medicine, 90033, Los Angeles, California

    Minnie McMillan

  3. Department of Microbiology, University of Texas Health Science Center at Dallas, 75235, Dallas, Texas

    James Forman

  4. Department of Neurology, University of Southern California School of Medicine, 90033, Los Angeles, California

    Peter R. Brayton

  5. Department of Microbiology and Immunology, University of North Carolina, 27514, Chapel Hill, North Carolina

    Jeffrey Frelingers

Authors
  1. Iwona Stroynowski
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  2. Anders Orn
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  3. Robert S. Goodenow
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  4. Minnie McMillan
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  5. James Forman
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  6. Peter R. Brayton
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  7. Jeffrey Frelingers
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  8. Leroy Hood
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Stroynowski, I., Orn, A., Goodenow, R.S. et al. Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by α1 and α2 but not α3 external domains. Immunogenetics 20, 141–154 (1984). https://doi.org/10.1007/BF00364486

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  • DOI: https://doi.org/10.1007/BF00364486

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