Abstract
Human alloimmune cytotoxic T cells, sensitized selectively against the HLA-A2 antigen, were tested on a panel of selected target cells. Five HLA-A2 positive outlier cells could be identified. These outlier cells were only weakly lysed by HLA-A2 specific CTLs, although they were serologically indistinguishable from the other HLA-A2 positive, strongly lysed target cells. Furthermore, it was found that the outlier cells were poor cold target inhibitors in contrast to the other HLA-A2 positive target cells, which showed adequate inhibition of specific lysis of HLA-A2 positive target cells. Population studies indicate that the frequency of such HLA-A2 outlier cells may be approximately 10%.
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Albrechtsen, D., Arnesen, E., and Thorsby, E.: Cell mediated lymphocytotoxicity directed against HLAD region products. Transplantation 27: 338–341, 1979
Biddison, W. E., Ward, F. E., Shearer, G. M., and Shaw, S.: The self determinants recognized by human virus immune T cells can be distinguished from the serologically defined HLA antigens. J. Immunol. 124: 548–552, 1980a
Biddison, W. E., Krangel, M. S., Strominger, J. L., Ward, F. E., Shearer, G. M., and Shaw, S.: Virus immune cytotoxic T cells recognize structural differences between serologically indistinguishable HLA-A2 molecules. Hum. Immunol. 3: 225–232, 1980b
Biddison, W. E., Shearer, G. M., and Shaw, S.: Influenza virus-specific cytotoxic T cells are restricted by multiple HLA-A3 related self antigens: evidence for recognition of distinct self structures in conjunction with different foreign antigens. J. Immunol. 127: 2231–2235, 1981
Bradley, B. A., Goulmy, E., Schreuder, L., and van Rood, J. J.: Targets for killer T cells in human lymphocyte differentiation. Its application to cancer. In B. Serrou and C. Rosenfeld (eds.): INSERM SYMPOSIUM No. 8, pp. 231–240. Elsevier/North Holland Biomedical Press, Amsterdam, 1978
Christiansen, F. T., Kim, S. J., Silver, D. M., and Dupont, B.: Cytotoxic effector cells against HLA antigens in strong linkage disequilibrium: identification of a strong, new CML determinant. Hum. Immunol. 2: 15–29, 1981
Eijsvoogel, V. P., Schellekens, P. T. A., du Bois, M. J. G., and Zeylemaker, W. P.: Human cytotoxic lymphocytes after alloimmunization in vitro. Transplant. Rev. 29: 125–163, 1976
Feighery, C. and Stastny, P.: HLA-D region associated determinants serve as targets for human cell mediated lympholysis. J. Exp. Med. 149: 485–494, 1979
Goulmy, E., Termijtelen, A., Bradley,B. A., and van Rood, J. J.: HLA restriction of non-HLA-A, -B, -C, and -D cell mediated lympholysis (CML). Tissue Antigens 8: 317–326, 1976
Goulmy, E., van Leeuwen, A., Blokland, E., van Rood, J. J., and Biddison, W. E.: MHC restricted H-Y specific antibodies and cytotoxic T lymphocytes can recognize different self determinants. J. Exp. Med. 155, 1567–1572, 1982
Grunnet, N., Kristensen, T., and Kissmeyer-Nielsen, F.: Cell mediated lympholysis in man. The impact of HLA-C antigens. Tissue Antigens 7: 301–309, 1976
Johnsen, H. E.: Human B-blast specific target determinants in CML. A methodological study. Tissue Antigens 15: 189–198, 1980
Kato, S., Iványi, P., Lacko, E., Breur, B., du Bois, R., and Eijsvoogel, V. P.: Identification of human CIVIL targets. HLA-B locus (1312) antigen variants defined by CTLs generated between B-locus-identical (B12) responder-stimulator pairs. J. Immunol. 128: 949–955, 1982
Kristensen, T., Grunnet, N., and Kissmeyer-Nielsen, F.: Cell mediated lympholysis in man. Occurrence of unexpected HLA-A (LA and Four) irrelevant lympholysis. Tissue Antigens 4: 378–382, 1974
Kristensen, T.: Human histocompatibility testing by T-cell mediated lympholysis. A European standard CML technique. Report from the European CML Study Group. Tissue Antigens 16: 335–367, 1980
Long, M. A., Handwerger, B. S., Amos, D. B., and Yunis, E. J.: The genetics of cell mediated lympholysis. J. Immunol. 117: 2092–2099, 1976
Mawas, C., Charmot, D., and Sasportes, M.: Is the LD region of the human MHC a CML target? In F. Kissmeyer-Nielsen (ed.): Histocompatibility Testing 1975, pp. 855–857, Munksgaard, Copenhagen, 1975
Melief, C. J. M., de Waal, L. P., van der Menlen, M. Y., Melvold, R. W., and Kohn,H. I.: Fine specificity of alloimmune cytotoxic T lymphocytes directed against H-2K. A study with Kb mutants. J. Exp. Med. 151: 993–1013, 1980
Miggiano, V. C., Bernoco, D., Lightbody, J., Trinchieri, G. B., and Ceppellini, R.: Cell mediated lympholysis in vitro with normal lymphocytes as targets. Specificity and cross-reactivity of the test. Transplant. Proc. 4: 231–237, 1972
Pfeffer, P. F. and Thorsby, E.: HLA-restricted cytotoxicity against male-specific (H-Y) antigen after acute rejection of an HLA-identical sibling kidney. Transplantation 33: 52–56, 1982
Robinson, M. A., Noreen, H. J., Amos, B. A., and Yunis, E. J.: Target antigens of cell mediated lympholysis discrimination of HLA subtypes by cytotoxic lymphocytes. J. Immunol. 121: 1486–1490, 1978
Schapira, M. and Jeannet, M.: Cell mediated lympholysis in HLA-A identical unrelated individuals. Tissue Antigens 4: 178–192, 1974
Schendel, D. J., Wank, R., and Dupont, B.: Cell mediated lympholysis: Examination of HLA genetic fine structure and complementation using cytotoxic lymphocytes. Eur. J. Immunol. 8: 634–640, 1978
Sherman, L. A.: Mutationally derived H-2 antigenic differences as defined by cytotoxic T lymphocytes clones. J. Immunol. 127: 1260–1269, 1981
Sondel, P. M. and Bach, F. H.: Recognitive specificity of human cytotoxic T lymphocytes: I. Antigen specific inhibition of human cell-mediated lympholysis. J. Exp. Med. 142: 1339–1348, 1975
Willumsen, J. and Heron, I.: Cell mediated lympholysis in man. A case of “non-relevant” killing of third parry persons. Tissue Antigens 4: 172–177, 1974
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Horai, S., van der Poel, J.J. & Goulmy, E. Differential recognition of the serologically defined HLA-A2 antigen by allogeneic cytotoxic T Cells. Immunogenetics 16, 135–142 (1982). https://doi.org/10.1007/BF00364400
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DOI: https://doi.org/10.1007/BF00364400