Skip to main content

Advertisement

SpringerLink
Log in

Mapping of class I DNA sequences within the human major histocompatibility complex

  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

Mutants that had lost expression of alleles of one or more HLA loci were isolated with immunoselection after γ-irradiation of a human lymphoblastoid cell line LCL 721. DNAs from the mutants were digested with restriction endonucleases and analyzed by Southern blotting using probes for class I HLA genes. Eight polymorphic cut sites for HindIII and PvuII were discovered in class I-associated sequences of LCL 721. Losses of specific fragments generated by restriction enzymes could be associated with losses of specific antigenic expressions and it was possible in this way to assign HLA-Al, HLA-A2, and HLA-B8 to specific DNA fragments. Patterns of γ-ray-induced segregations of DNA fragments permitted rough linkage alignment of about 30% of the fragments generated by PvuII. The resultant map showed that there are class I HLA genes on the telomeric side of the HLA-A locus. Restriction enzyme site polymorphisms were also examined in a panel of DNAs isolated from peripheral blood lymphocytes (PBLs) of HLA-typed individuals. This panel of PBL DNA complemented the analysis using the HLA deletion mutants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abrahamson, S. and Wolff, S.: Reanalysis of radiation-induced specific locus mutations in the mouse. Nature 264: 715–719, 1976

    Google Scholar 

  • Barbosa, J. A., Kamarck, M. E., Biro, P. A., Weissman, S. M., and Ruddle, F. H.: Identification of human genomic clones coding the major histocompatibility antigens HLA-A2 and HLA-B7 by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. U.S.A. 79: 6327–6331, 1982

    Google Scholar 

  • Berger, R., Bernheim, A., Sasportes, M., Hauptmann, G., Hors, J., Legrand, L., and Fellous, M.: Regional mapping of the HLA on the short arm of chromosome 6. Clin. Genet. 15: 245–251, 1979

    Google Scholar 

  • Biro, P. A., Reddy, V. B., Sood, A., Pereira, D., and Weissman, S. M.: Isolation and analysis of human major histocompatibility complex genes. In A. G. Walton (ed.): Recombinant DNA, pp. 41–49, Elsevier Amsterdam, 1981

  • Blin, H. and Stafford, D. W.: A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 3: 2303–2308, 1976

    Google Scholar 

  • Boyum, A.: Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Invest. 21: 77–89, 1968

    Google Scholar 

  • Cami, B., Bregegere, F., Abastado, J. P., and Kourilsky, P.: Multiple sequences related to classical histocompatibility antigens in the mouse genome. Nature 291: 673–675, 1981

    Google Scholar 

  • Christensen, R.: Specificity and frequency of ultraviolet-induced reversion of an iso-l-cytochrome C ochre mutants in radiation-sensitive strains of yeast. J. Mol. Biol. 85: 137–162, 1974

    Google Scholar 

  • Cosman, D., Khoury, G., and Jay, G.: Three classes of mouse H-2 messenger RNA distinguished by analysis of cDNA clones. Nature 295: 73–76, 1982

    Google Scholar 

  • DeMars, R., Chang, C. C., and Rudersdorf, R.: Dissection of the D-region of the human major histocompatibility complex by means of induced mutations in a lymphoblastoid cell line. Hum. Immunol., in press 1983

  • Evans, G. A., Margulies, D. H., Camerini-Otero, R. D., Ozato, K., and Seidman, S. G.: Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld. Proc. Natl. Acad. Sci. U.S.A. 79: 1994–1998, 1982

    Google Scholar 

  • Goodenow, R. S., McMillan, M., Orn, A., Nicolson, M., Davidson, N., Frelinger, J. A., and Hood, L.: Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. Science 215: 677–679, 1982

    Google Scholar 

  • Goodfellow, P. W., Jones, E. A., van Hegringen, J., Soloman, E., Bobrow, M., Miggiano, U., and Bodmer, W. F.: The β2-microglobulin gene is in chromosome 15 and not in the HL-A region. Nature 254: 267–269, 1975

    Google Scholar 

  • Kavathas, P., Bach, F. H., and DeMars, R.: Gamma-ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells. Proc. Natl. Acad. Sci. U.S.A. 77: 4251–4255, 1980

    Google Scholar 

  • Kvist, S., Bregegere, F., Rask, L., Cami, B., Garoff, H., Daniel, F., Wiman, K., Larhammar, D., Abastado, J. P., Gachelin, G., Peterson, P. A., Dobberstein, B., and Kourilsky, P.: cDNA clone coding for part of a mouse H-2d major histocompatibility antigen. Proc. Natl. Acad. Sci. U.S.A. 78: 2772–2776, 1981

    Google Scholar 

  • Lemonnier, F. A., Malissen, M., Godstein, P., Le-Bouteiller, P., Rabai, N., Damotte, M., Birnbaum, D., Caillol, D., Truey, J., and Jordan, B. R.: Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L cells. Immunogenetics 16: 355–361, 1982

    Google Scholar 

  • Malissen, M., Malissen, B., and Jordan, B. R.: Exon/intron organization and complete nucleotide sequence of an HLA gene. Proc. Natl. Acad. Sci. U.S.A. 79: 893–897, 1982a

    Google Scholar 

  • Malissen, M., Damotte, M., Birnbaum, D., Truey, J., and Jordan, B.R.: HLA cosmid clones show complete, widely spaced human class I genes with occasional clusters. Gene 20: 485–489, 1982b

    Google Scholar 

  • Margulies, D. H., Evans, G. A., Flaherty, L., and Seidman, J. G.: H-2 like genes in the Tla region of mouse chromosome 17. Nature 295: 168–170, 1982

    Google Scholar 

  • Mellor, A. L., Golden, L., Weiss, E., Bullman, H., Hurst, J., Simpson, E., James, R., Townsend, A. R. M., Taylor, P. M., Schmidt, W., Ferluga, J., Leben, L., Santamaria, M., Atfield, G., Festenstein, H., and Flavell, R. A.: Expression of murine H-2kb histocompatibility antigen in cells transformed with cloned H-2 genes. Nature 298: 529–534, 1982

    Google Scholar 

  • Moore, K. W., Sher, B. T., Sun, Y. H., Eakle, K. A., and Hood, L.: DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen. Science 215: 679–682, 1982

    Google Scholar 

  • Orr, H. T.: The major histocompatibility complex: Analysis at the protein and DNA levels. In P. Parham and J. Strominger (eds.): Histocompatibility Antigens, Structure and Function, pp. 1–52, Chapman and Hall, London, 1982

    Google Scholar 

  • Orr, H. T. and DeMars, R.: Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells. Nature 302: 534–536, 1983

    Google Scholar 

  • Orr, H. T., Bach, F. H., Ploegh, H. L., Strominger, J. L., Kavathas, P., and DeMars, R.: Use of HLA loss mutants to analyze the structure of the human major histocompatibility complex. Nature 296: 454–456, 1982

    Google Scholar 

  • Parham, P. and Brodsky, F. M.: Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277–299, 1981

    Google Scholar 

  • Pease, L. R., Nathenson, S. G., and Leinwand, L. A.: Mapping class I gene sequences in the major histocompatibility complex. Nature 298: 382–385, 1982

    Google Scholar 

  • Ploegh, H. L., Orr, H. T., and Strominger, J. L.: Molecular cloning of a human histocompatibility antigen cDNA fragment. Proc. Natl. Acad. Sci. U.S.A. 77: 6081–6085, 1980

    Google Scholar 

  • Rigby, P. W. J., Dieckmann, M., Rhodes, C., and Berg, P.: Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. I. J. Mol. Biol. 113: 237–251, 1977

    Google Scholar 

  • Sood, A. V., Pereira, D., and Weissman, S. M.: Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc. Natl. Acad. Sci. U.S.A. 78: 616–620, 1981

    Google Scholar 

  • Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517, 1975

    Google Scholar 

  • Steinmetz, M., Frelinger, J. G., Fisher, D., Hunkapiller, T., Pereira, D., Weissmann, S. M., Uehara, H., Nathenson, S., and Hood, L.: Three cDNA clones encoding mouse transplantation antigens: Homology to immunoglobulm genes. Cell 24: 125–134, 1981a

    Google Scholar 

  • Steinmetz, M., Moore, K. W., Frelinger, J. G., Sher, B. T., Shen, F.-W., Boyse, E. A., and Hood, L.: A pseudogene homologous to mouse transplantation antigens: Transplantation antigens are encoded by eight exons that correlate with protein domains. Cell 25: 683–692, 1981b

    Google Scholar 

  • Steinmetz, M., Winoto, A., Minard, K., and Hood, L.: Clusters of genes encoding mouse transplantation antigens. Cell 28: 489–498, 1982

    Google Scholar 

  • Wahl, G. M., Stern, M., and Stark, G. R.: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxy methyl-paper and rapid hybridization by using dextran sulfate. Proc. Natl. Acad. Sci. U.S.A. 76: 3683–3687, 1979

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Laboratory Medicine/Pathology and Department of Microbiology, University of Minnesota, 55455, Minneapolis

    Harry T. Orr

  2. Laboratory of Genetics and Department of Human Oncology, University of Wisconsin, 53706, Madison, Wisconsin

    Robert DeMars

Authors
  1. Harry T. Orr
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert DeMars
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orr, H.T., DeMars, R. Mapping of class I DNA sequences within the human major histocompatibility complex. Immunogenetics 18, 489–502 (1983). https://doi.org/10.1007/BF00364390

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00364390

Keywords

Search

Navigation