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Identification of several functional subgroups of HLA-B27 by restriction of the activity of antiviral T killer lymphocytes

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Abstract

Anti-Epstein-Barr virus and antiinfluenza A cytotoxic T lymphocytes (CTL) have been used to study the restriction of human antiviral responses by HLA-B27 antigens. Three functional subgroups of HLA-B27 have been clearly distinguished by this “restriction-typing assay”. No cross-reaction could be detected between the three subgroups either at the CTL level or at the level of antigen-presenting cells. The cells of subgroup 1 are always positive [M2(+)] when tested in immunofluorescence with a monoclonal B27-specific antibody which divides HLA-B27 into a major M2(+) and a minor M2(−) subgroup. These M2(+) group 1 cells are apparently also HLA-B27W as previously shown by Ivanyi and co-workers using anti-HLA-CTL. Subgroup 2 includes only M2(−) cells. A comparison between this group and the previously described HLA-B27K is not fully conclusive, since two typing cells which were clearly HLA-B27K apparently did not belong to group 2. Only two donors, both of Oriental origin, have been included in subgroup 3. Both of them were “M2 intermediate”. These results demonstrate (1) the existence of several functional subgroups of HLA-B27 with an interesting correlation with the M2(+), M2(−), or M2 intermediate phenotypes, and (2) the possibility of using the restriction-typing assay to define such functional subgroups not detected by classical allosera.

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Abbreviations

CTL:

cytolytic T lymphocyte

EBV:

Epstein-Barr virus

AS:

ankylosing spondylarthritis

PBL:

peripheral blood leukocytes

MSL:

medium for separation of lymphocyte

UV:

ultra violet

PHA-M:

phytohemagglutinin M

LCL:

lymphoblastoid cells lines

FCS:

fetal calf serum

CRT:

chromium release test

SCR:

specific chromium release

LU:

lytic units

MoAb:

monoclonal antibodies.

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Authors and Affiliations

  1. Laboratoire d'Immunologie et de Virologie des Tumeurs, INSERM U.152, Hôpital Cochin, 27 rue du Faubourg Saint-Jacques, 75674, Paris cedex 14, France

    Antoine Toubert, Elisabeth Gomard & Jean-Paul Levy

  2. Stanford University Medical Center, Stanford University School of Medicine, 94305, Stanford, California

    F. Carl Grumet

  3. Clinique de Rhumatologie, Hôpital Cochin, 27 rue du Faubourg Saint-Jacques, 75674, Paris cedex 14, France

    Bernard Amor

  4. Centre National de Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France

    Jean-Yves Muller

Authors
  1. Antoine Toubert
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  2. Elisabeth Gomard
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  3. F. Carl Grumet
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  4. Bernard Amor
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  5. Jean-Yves Muller
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  6. Jean-Paul Levy
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Toubert, A., Gomard, E., Grumet, F.C. et al. Identification of several functional subgroups of HLA-B27 by restriction of the activity of antiviral T killer lymphocytes. Immunogenetics 20, 513–525 (1984). https://doi.org/10.1007/BF00364354

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  • DOI: https://doi.org/10.1007/BF00364354

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