Abstract
In a preceding report, the detection of an H-2-linked immune response to the H-Xd antigen on the P815-X2 mastocytoma was demonstrated by the significantly increased survival of (C57BL/6 × DBA/2)F1 (B6D2F1) male hybrids (H-X b) compared with female siblings (H-X b/H-X d) after injection with the histocompatible tumor (H-X d). This interpretation was supported by the absence of this sex effect in reciprocal D2B6F1 hybrids (H-X d and H-X d/H-X b). Additional findings presented in this paper support the conclusion that this sex effect is due to a true immunological response to H-Xd: (a) Reciprocal (DBA/2 × C57BL/6 H-2 mutant)F1 hybrids, as well as D2B6F1, failed to exhibit the sex effect: (b) the demonstration of the sex effect in (BALB/c × DBA/2)F1 and (BALB/c-H-2 dm2 × DBA/2)F1 hybrids and in (C57BL/10 × DBA/2)F1 hybrids was consistent with the known H-X incompatibilities between the strains BALB/c and DBA/2 and C57BL/10 and DBA/2, respectively, previously demonstrated by skin grafting; and (c) the sex effect was not abrogated by castration of male B6D2F1 hybrids. Variability in the presence or absence of the sex effect was observed in various [recombinant inbred (RI) × DBA/2]F1 hybrids and may be attributed to the influence of a regulatory non-H-2 gene which is closely linked to the gene coding for mouse kidney-androgen-regulated protein (KAP) but androgen-independent, or to variability in inheritance of the H-X b allele among the RI lines. It is proposed that the P815-X2 model may be utilized to type RI lines derived from a cross between C57BL/6 and DBA/2 for their H-X genotypes.
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Abbreviations
- B:
-
C57BL/6 origin allele
- B6:
-
C57BL/6
- B10:
-
C57BL/10
- B6D2F1:
-
(C57BL/6 × DBA/2)F1
- B6mD2F1:
-
(C57BL/6 H-2 mutant × DBA/2)F1
- bm10 :
-
B6.C-H-2 bm10
- C:
-
BALB/c
- D:
-
DBA/2 origin allele
- D2:
-
DBA/2
- dm2 :
-
BALB/c-H-2 dm2
- H-X:
-
X chromosome-determined histocompatibility antigen of the mouse
- Ir :
-
gene, immune response gene
- KAP:
-
kidney androgenregulated protein
- MST:
-
median survival time
- RI:
-
recombinant inbred
- SDP:
-
strain distribution pattern
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Kwak, L.W., Melvold, R.W. & Williams, R.M. Immune response to the H-X antigen on P815-X2. Immunogenetics 20, 481–491 (1984). https://doi.org/10.1007/BF00364351
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DOI: https://doi.org/10.1007/BF00364351