Abstract
Antibody diversity, as measured by isoelectric focusing of dinitrophenol-specific antibodies, was compared in different polyploid species of the clawed toad Xenopus. Antibody heterogeneity increased with chromosome number and DNA content from Xenopus tropicalis (2n=20 chromosomes) to Xenopus ruwenzoriensis (2n=108 chromosomes). Laboratory allopolyploids made by hybridization between two species showing different antibody diversities and different chromosome numbers gave antibody patterns intermediate between the two parents. On the other hand, autopolyploid individuals showed no increase in antibody diversity, showing that increased polyploidy alone cannot be responsible for increased heterogeneity. In contrast to the increase in antibody diversity following polyploidization, the number of expressed major histocompatibility complex alleles, as measured by a mixed lymphocyte reaction, did not increase. This locus appeared to be diploid or in the process of rediploidization in all the Xenopus species studied. Selection has thus operated differentially on the polyploid immunoglobulin and major histocompatibility loci. It apparently preserved the additional heterogeneity acquired for immunoglobulins favoring the expression of an expanded antibody repertoire in polyploid species.
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Avtalion, R. R., Wojdani, A., Malik, Z., Shahrabani, R., and Duczyminer, M.: Influence of environmental temperature on the immune response in fish. Curr. Topics. Microbiol. Immunol. 61: 1–36, 1973
Bernard, C. C. A., Bordmann, G., Blomberg, B., and Du Pasquier, L.: Immunogenetic studies on the cell-mediated cytotoxicity in the clawed toad Xenopus laevis. Immunogenetics 9: 443–454, 1979
Bernard, C. C. A., Bordmann, G., Blomberg, B., and Du Pasquier, L.: Genetic control of T helper cell function in the clawed toad Xenopus laevis. Eur. J. Immunol. 11: 151–155, 1981
Bisbee, C. A., Baker, M. A., Wilson, A. C., Fischberg, M., and Hadji-Azimi, I.: Albumin phylogeny for clawed frogs (Xenopus). Science 195: 785–787, 1977
Blomberg, B., Bernard, C. C. A., and Du Pasquier, L.: In vitro evidence for T-B lymphocyte collaboration in the clawed toad Xenopus. Eur. J. Immunol. 10: 869–876, 1980
Brandt, D. C., Griessen, M., Du Pasquier, L., and Jaton, J. C.: Antibody diversity in amphibians: evidence for the inheritance of idiotypic specificities in isogenic Xenopus. Eur. J. Immunol. 10: 731–736, 1980
Du Pasquier, L., Weiss, N., and Loor, F.: Direct evidence for immunoglobulins on the surface of thymus lymphocytes of amphibian larvae. Eur. J. Immunol. 2: 366–370, 1972
Du Pasquier, L., Chardonnens, X., and Miggiano, V. C.: A major histocompatibility complex in the toad Xenopus laevis (Daudin). Immunogenetics 1: 482–494, 1975
Du Pasquier, L., Miggiano, V. C., Kobel, H. R., and Fischberg, M.: The genetic control of histocompatibility reactions in natural and laboratory-made polyploid individuals of the clawed toad Xenopus. Immunogenetics 5: 129–141, 1977
Du Pasquier, L. and Wahl, M. R.: Antibody diversity in amphibians: inheritance of isoelectric focusing patterns in isogenic frogs. Eur. J. Immunol. 8: 428–433, 1978
Du Pasquier, L. and Kobel, H. R.: Histocompatibility antigens and immunoglobulin genes in the clawed toad: expression and linkage studies in recombinant and hyperdiploid Xenopus hybrids. Immunogenetics, 8: 299–310, 1979
Elkan, E.: Amphibians. In A. N. Worden and W. Lane-Petter (eds.): UFAW Handbook of the Care and Management of Laboratory Animals, 2nd edit., pp. 804–815, Livingstone, Edinburgh, 1957
Fischberg, M. and Kobel, H. R.: Two new polyploid Xenopus species from Western Uganda. Experientia 34: 1012–1014, 1978
Haimovich, J. and Du Pasquier, L.: Specificity of antibodies in amphibian larvae possessing a small number of lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 70: 1898–1902, 1973
Hozumi, N. and Tonegawa, S.: Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions. Proc. Natl. Acad. Sci. 73: 3628–3632, 1976
Jeffreys, A. J., Wilson, V., Wood, D., Simons, J. P., Kay, R. M., and Williams, J. G.: Linkage of adult α and β globin genes in X laevis and gene duplication by tetraploidization. Cell 21: 555–564, 1980
Kobel, H. R. and Du Pasquier, L.: Production of large clones of histocompatible, fully identical clawed toads (Xenopus). Immunogenetics 2: 87–91, 1975
Kobel, H. R. and Du Pasquier, L.: Strains and species of Xenopus for immunological research. In J. B. Solomon and J. D. Horton (eds.): Developmental Immunobiology, pp. 299–306, Elsevier/North Holland, Amsterdam, 1977
Jerne, N. K.: The somatic generation of immune recognition. Eur. J. Immunol. 1: 1–9, 1971
Phillipps, J. M. and Dresser, D. W.: Antibody isoelectric spectra visualized by antigen-coated erythrocytes. Eur. J. Immunol. 3: 738–740, 1973
Tata, J. R., Baker, B. S., and Deeley, J. V.: Vitellogenin as a multigene family. J. Biol. Chem. 255: 6721–6726, 1980
Tinsley, R. C., Kobel, H. R., and Fischberg, M.: The biology and systematics of a new species of Xenopus (Amura Pipidac) from the highlands of Central Africa. J. Zool. Lond. 188: 69–102, 1979
von Boehmer, H., Haas, W., and Jerne, N. K.: Major histocompatibility complex-linked immune responsiveness is acquired by lymphocytes of low-responder mice differentiating in thymus of high-responder mice. Proc. Natl. Acad. Sci. U.S.A. 75: 2439–2442, 1978
Wabl, M. R. and Du Pasquier, L.: Antibody patterns in genetically identical frogs. Nature 264: 642–644, 1976
Wahli, W., Dawid, I. B., Wyler, T., Weber, R., and Ryffel, G. V.: Comparative analysis of the structural organization of two closely related vitellogenin genes in X. laevis. Cell 20: 107–117, 1980
Weigert, M. G., Cesari, I. M., Yonkovich, S. J., and Cohn, M.: Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045–1050, 1970
Weiss, N. and Du Pasquier, L.: Factors affecting the reactivity of amphibian lymphocytes in a miniaturized technique of the mixed lymphocyte culture. J. Immunol. Methods 3: 273–286, 1973
Weiss, N., Horton, J. D., and Du Pasquier, L.: The effect of thymectomy on cell surface associated and serum immunoglobulin in the toad Xenopus laevis (Daudin): a possible inhibitory role of the thymus on the expression of immunoglobulins. In J. Panijel and P. Liacopoulos (eds.): L'Etude Plylogénique et Ontigénique de la Réponse Immunitaire et son Apport a la Théorie Immunologique, pp. 165–174, INSERM, Paris, 1973
Westley, B., Wyler, T., Ryffel, G., and Weber, R.: Xenopus laevis serum albumins are encoded in two closely related genes. Nucleic Acids Res. 9: 3557–3574, 1981
Zinkernagel, R. M., Callahan, G. N., Klein, J., and Dennert, G.: Cytotoxic T cells learn specicity for self H-2 during differentiation in the thymus. Nature 271: 251–253, 1978
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Du Pasquier, L., Blomberg, B. The expression of antibody diversity in natural and laboratory-made polyploid individuals of the clawed toad Xenopus . Immunogenetics 15, 251–260 (1982). https://doi.org/10.1007/BF00364333
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DOI: https://doi.org/10.1007/BF00364333