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A new murine lymphocyte alloantigen, Ly-21.2, mapping to the seventh chromosome

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Abstract

Using a monoclonal antibody raised by fusing spleen cells from A/J mice, immunized with B10.A splenocytes and lymph-node cells, with a BALB/c myeloma, we have described a new surface alloantigen, Ly-21.2. Ly-21.2 is present in varying amounts in all lymphoid tissues, is not detectable in the brain, kidney, lung or erythrocytes, and is found in only trace amounts in the liver. Strain distribution studies showed that Ly-21.2 is present in all strains examined, including B10, except the A strain and segregation analysis of (A/J × 1310) F2 mice showed that Ly-21.2 expression (1) is encoded by one gene and (2) is linked to albinism on chromosome 7. Studies performed on mice developing T-cell leukemia showed that, regardless of the etiologic agent, Ly-21.2 expression increases dramatically in mice with overt leukemia. In addition, preliminary studies suggest that expression of Ly-21.2 is linked to increased susceptibility of mice to Friend-virus-induced erythroleukemia.

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Abbreviations

B10:

C57BL/10

CPM:

counts per minute

FFU:

focus-forming units

FV:

Friend virus

i.v.:

intravenous

NMS:

normal mouse serum

PBS:

phosphate-buffered saline

RAMIG:

rabbit anti-mouse IgG

RIA:

radioimmunoassay

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Authors and Affiliations

  1. Irvington House Institute, Department of Medicine, New York University Medical Center, 550 First Avenue, 10016, New York, New York

    Janis Kennard & Daniel Meruelo

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  1. Janis Kennard
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  2. Daniel Meruelo
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Kennard, J., Meruelo, D. A new murine lymphocyte alloantigen, Ly-21.2, mapping to the seventh chromosome. Immunogenetics 15, 239–250 (1982). https://doi.org/10.1007/BF00364332

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  • DOI: https://doi.org/10.1007/BF00364332

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