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Fine specificity of a proliferating T-cell clone activated by a conformational determinant of the I-Ek molecule

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Abstract

We have examined the fine specificity of a stable Thy-1.2+, Lyt-1.2+, Lyt-2−, and I-As− anti-I-Ek proliferating T-cell clone isolated from an A.TH anti-A.TL secondary mixed lymphocyte culture. Spleen cells from various I-Ak, Ek strains induced either a strong (A.TL, OH, and CBA) or a weak (AKR and B10.BR) proliferative response, although such cells expressed at their surface similar amounts of I-Ek antigens. Analysis of H-2 recombinant strains indicated that this clone recognized a conformational determinant carried by the E kβ E kα dimer, but not on the Ea chain per se. Among the Fl hybrid strains in which the combinatorial E kβ E kα product was detected by cellular binding with monoclonal E kβ -specific antibodies (mAb), some [(BIO.S(8R) × BlO.HTT) but not others (for example, B10.A(4R) × B10.A(5R)] were stimulatory. Seventeen anti-Ek mAb, regardless of the three spatially separated domains that they defined by antibody binding competition, completely inhibited the restimulation of this clone, whereas 15 other anti-Ak mAb failed to do so. This clone was not reactivated by stimulating cells from strains with the H-2 haplotypes p, j, v, b, r, and s but it proliferated strongly against cells from several H-2 d or H-2 q strains. Genetic evidence or blocking studies with selected mAb assigned these cross-reactive mixed lymphocyte reaction determinants to the Ad or Aq molecules, respectively. The data support the conclusion that alloreactive T cells may define a polymorphism of I-region coded products not detected by serological analyses and extend at the T-cell level the observations of serological cross-reactions between A and E molecules.

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  1. Centre d'Immunologie, INSERM-CNRS de Marseille-Luminy, Case 906, 13288, Marseille Cédex 9, France

    Anne Pierres & Michel Pierres

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  1. Anne Pierres
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Pierres, A., Pierres, M. Fine specificity of a proliferating T-cell clone activated by a conformational determinant of the I-Ek molecule. Immunogenetics 15, 399–412 (1982). https://doi.org/10.1007/BF00364263

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